Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tau (phospho S199) antibody [EPR2401Y]

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate 10 mM, tween 20 0.5%, pH =6
Permeabilization
Yes - triton 0.75%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: DK serum 5%
Dilution
1/100

Secondary antibody

Dilution
1/250

Mrs. Francoise Geffroy

Verified customer

Submitted Mar 28 2018

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