Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S202) antibody [EPR2402]

Inconclusive
Abreviews
This product is known to not work in this application or species.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Tau/APP transgenic mouse expressing human Tau)
Specification
Tau/APP transgenic mouse expressing human Tau
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/1500

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted composite image shows no obvious neurones with NFTs (upper image) whereas they are clearly seen using another antibody ( lower image). Both images are near serial sections from a Tau/APP transgenic mouse.
Abcam response
Thank you for you valuable feedback. It appears that under these experimental conditions ab108387 does not specifically detect human Tau phosphorylated at Serine 202 in transgenic mice. We welcome further customer comments. For more information, please contact scientific support.

Mr. Carl Hobbs

Verified customer

Submitted Dec 12 2013

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