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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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Anti-Tau (phospho S238) antibody [12G10] (ab128889)

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Western blot - Anti-Tau (phospho S238) antibody [12G10] (ab128889)

    Key features and details

    • Mouse monoclonal [12G10] to Tau (phospho S238)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Tau (phospho S238) antibody [12G10]
      See all Tau primary antibodies
    • Description

      Mouse monoclonal [12G10] to Tau (phospho S238)
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      corresponding to Tau.

    • General notes

      This antibody clone is manufactured by Abcam.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      12G10
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • MT Associated Proteins
      • Tau
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Axon marker

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant human Tau (mutated P301S) protein (Active) (ab246005)
    • Related Products

      • Human Tau ELISA Kit (ab210972)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab128889 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/200. Predicted molecular weight: 79 kDa.
    Notes
    WB
    1/200. Predicted molecular weight: 79 kDa.

    Target

    • Function

      Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
    • Tissue specificity

      Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
    • Involvement in disease

      Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
      Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
      Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
      Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
      Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
    • Sequence similarities

      Contains 4 Tau/MAP repeats.
    • Developmental stage

      Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
    • Domain

      The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
    • Post-translational
      modifications

      Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
      Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
      Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
    • Cellular localization

      Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
    • Target information above from: UniProt accession P10636 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4137 Human
      • Omim: 157140 Human
      • SwissProt: P10636 Human
      • Unigene: 101174 Human
      • Form

        There are 9 isoforms produced by alternative splicing.
      • Alternative names

        • AI413597 antibody
        • AW045860 antibody
        • DDPAC antibody
        • FLJ31424 antibody
        • FTDP 17 antibody
        • G protein beta1/gamma2 subunit interacting factor 1 antibody
        • MAPT antibody
        • MAPTL antibody
        • MGC134287 antibody
        • MGC138549 antibody
        • MGC156663 antibody
        • Microtubule associated protein tau antibody
        • Microtubule associated protein tau isoform 4 antibody
        • Microtubule-associated protein tau antibody
        • MSTD antibody
        • Mtapt antibody
        • MTBT1 antibody
        • MTBT2 antibody
        • Neurofibrillary tangle protein antibody
        • Paired helical filament tau antibody
        • Paired helical filament-tau antibody
        • PHF tau antibody
        • PHF-tau antibody
        • PPND antibody
        • PPP1R103 antibody
        • Protein phosphatase 1, regulatory subunit 103 antibody
        • pTau antibody
        • RNPTAU antibody
        • TAU antibody
        • TAU_HUMAN antibody
        • Tauopathy and respiratory failure antibody
        • Tauopathy and respiratory failure, included antibody
        see all

      Images

      • Western blot - Anti-Tau (phospho S238) antibody [12G10] (ab128889)
        Western blot - Anti-Tau (phospho S238) antibody [12G10] (ab128889)

        Immunoblot analysis of ab128889, at 1:200 dilution, performed in transgenic flies expressing human Tau.  This antibody recognises wild-type human Tau (isoforms 0N4R and 2N4R) but not the mutant (STA), where Ser238 has been replaced by a non-phosphorylatable Alanine.

         

          

         

         

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (2)

      Publishing research using ab128889? Please let us know so that we can cite the reference in this datasheet.

      ab128889 has been referenced in 2 publications.

      • Ercan-Herbst E  et al. A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain. Acta Neuropathol Commun 7:192 (2019). PubMed: 31796124
      • Ercan E  et al. A validated antibody panel for the characterization of tau post-translational modifications. Mol Neurodegener 12:87 (2017). WB, PepArr ; Human . PubMed: 29157277

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