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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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RecombinantRabMAb

Recombinant Anti-Tau (phospho S396) antibody [E178] (ab32057)

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Reviews (5)Q&A (2)References (45)

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Western blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunoprecipitation - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Dot Blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • ELISA - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Western blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Affinity Purification - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
  • Anti-Tau antibody [E178] (ab32057)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E178] to Tau (phospho S396)
  • Suitable for: IHC-P, Dot blot, ELISA, IHC-Fr, WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Tau (phospho S396) antibody [E178]
    See all Tau primary antibodies
  • Description

    Rabbit monoclonal [E178] to Tau (phospho S396)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Dot blot, ELISA, IHC-Fr, WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse brain tissue lysates; IP: Human fetal brain lysates; IHC-P: Human cerebrum and salivary gland; Mouse colon and rat colon and tongue tissue; IHC-Fr: Mouse and Rat cerebrum tissue, Human Alzheimer brain tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E178
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Development
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Tau (phospho S396) antibody [E178] - BSA and Azide free (ab218600)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/1000 - 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat. The mouse and rat recommendation is based on other applications.

AP
Use at an assay dependent concentration.

Antibody concentration range - 3.33, 1.67, 0.83, 0.42, 0.21, 0 nM/mL

Dot blot
1/1000.
ELISA
Use at an assay dependent concentration.
IHC-Fr (1)
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

WB (2)
1/1000. Predicted molecular weight: 79 kDa.

For unpurified use at 1/5000

IP
1/20.

For unpurified use at 1/100

Notes
IHC-P
1/1000 - 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat. The mouse and rat recommendation is based on other applications.

AP
Use at an assay dependent concentration.

Antibody concentration range - 3.33, 1.67, 0.83, 0.42, 0.21, 0 nM/mL

Dot blot
1/1000.
ELISA
Use at an assay dependent concentration.
IHC-Fr
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

WB
1/1000. Predicted molecular weight: 79 kDa.

For unpurified use at 1/5000

IP
1/20.

For unpurified use at 1/100

Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • Tissue specificity

    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
  • Involvement in disease

    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
  • Sequence similarities

    Contains 4 Tau/MAP repeats.
  • Developmental stage

    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
  • Domain

    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
  • Post-translational
    modifications

    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
  • Cellular localization

    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Target information above from: UniProt accession P10636 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281296 Cow
    • Entrez Gene: 4137 Human
    • Entrez Gene: 17762 Mouse
    • Entrez Gene: 29477 Rat
    • Omim: 157140 Human
    • SwissProt: P29172 Cow
    • SwissProt: P10636 Human
    • SwissProt: P10637 Mouse
    • SwissProt: P19332 Rat
    • Unigene: 101174 Human
    • Unigene: 1287 Mouse
    • Unigene: 2455 Rat
    see all
  • Form

    There are 9 isoforms produced by alternative splicing.
  • Alternative names

    • AI413597 antibody
    • AW045860 antibody
    • DDPAC antibody
    • FLJ31424 antibody
    • FTDP 17 antibody
    • G protein beta1/gamma2 subunit interacting factor 1 antibody
    • MAPT antibody
    • MAPTL antibody
    • MGC134287 antibody
    • MGC138549 antibody
    • MGC156663 antibody
    • Microtubule associated protein tau antibody
    • Microtubule associated protein tau isoform 4 antibody
    • Microtubule-associated protein tau antibody
    • MSTD antibody
    • Mtapt antibody
    • MTBT1 antibody
    • MTBT2 antibody
    • Neurofibrillary tangle protein antibody
    • Paired helical filament tau antibody
    • Paired helical filament-tau antibody
    • PHF tau antibody
    • PHF-tau antibody
    • PPND antibody
    • PPP1R103 antibody
    • Protein phosphatase 1, regulatory subunit 103 antibody
    • pTau antibody
    • RNPTAU antibody
    • TAU antibody
    • TAU_HUMAN antibody
    • Tauopathy and respiratory failure antibody
    • Tauopathy and respiratory failure, included antibody
    see all

Images

  • Western blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Western blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    All lanes : Anti-Tau (phospho S396) antibody [E178] (ab32057) at 1/1000 dilution

    Lane 1 : Human brain lysates
    Lane 2 : Human brain lysates and the membrane was incubated with alkaline phosphatase
    Lane 3 : Human brain lysates and the membrane was incubated with lambda phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50-79 kDa why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Immunohistochemistry analysis of paraffin-embedded human cerebrum tissue sections labeling Tau (phospho S396) with ab32057 at 1/4000 dilution (0.026 μg/mL). Goat Anti-Rabbit IgG H&L (HRP polymer) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on human cerebrum without alkaline phosphatase treatment (image A). No staining on human cerebrum with alkaline phosphatase treatment (image B).
    The section was incubated with ab32057 overnight at +4°C.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Immunohistochemistry analysis of frozen mouse cerebrum tissue sections labeling Tau (phospho S396) with ab32057 at 1/100 (1μg/mL). ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Cytoplasmic staining on mouse cerebrum, the signal decreased after phosphatase treatment at 37℃ for 2h.

  • Immunoprecipitation - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunoprecipitation - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    ab32057 (purified) at 1/20 dilution (0.5ug) immunoprecipitating Tau in Human fetal brain lysates.
    Lane 1: Human fetal brain lysates 10ug
    Lane 2 (+): ab32057 & Human fetal brain lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32057 in Human fetal brain lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Dot Blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Dot blot analysis of Tau (phospho S396) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (phospho S396) with ab32057 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • ELISA - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    ELISA - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Indirect ELISA antigen dose-response curve using ab32057 at 1000-0 ng/mL. Antigen Human Tau (phospho S396) peptide, Human Tau non-phospho peptide at concentration of 1000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG H+L at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Western blot - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    All lanes : Anti-Tau (phospho S396) antibody [E178] (ab32057) at 1/1000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse brain lysates and the membrane was incubated with alkaline phosphatase
    Lane 3 : Mouse brain lysates and the membrane was incubated with lambda phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50-79 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Immunohistochemistry analysis of paraffin-embedded mouse colon tissue sections labeling Tau (phospho S396) with ab32057 at 1/4000 dilution (0.026 μg/mL). Goat Anti-Rabbit IgG H&L (HRP polymer) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on ganglions of mouse colon without alkaline phosphatase treatment (image A). No staining on ganglions of mouse colon with alkaline phosphatase treatment (image B).
    The section was incubated with ab32057 overnight at +4°C.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Immunohistochemistry analysis of frozen rat cerebrum tissue sections labeling Tau (phospho S396) with ab32057 at 1/100 (1 μg/mL). ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Cytoplasmic staining on rat cerebrum, the signal decreased after phosphatase treatment at 37℃ for 2h.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Immunohistochemistry analysis of paraffin-embedded rat colon tissue sections labeling Tau (phospho S396) with ab32057 at 1/4000 dilution (0.026 μg/mL). Goat Anti-Rabbit IgG H&L (HRP polymer) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on ganglions of rat colon without alkaline phosphatase treatment (image A). No staining on ganglions of rat colon with alkaline phosphatase treatment (image B).
    The section was incubated with ab32057 overnight at +4°C.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    IHC image of Tau staining in a section of frozen normal human Alzheimer brain performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab32057, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Affinity Purification - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Affinity Purification - Anti-Tau (phospho S396) antibody [E178] (ab32057)

    Biotinylated Human Tau (pS396) [0.05 µg/ml] was loaded to SA biosensor on Fortebio RED96e Machine, then associate with recombinant Anti-Tau (phospho S396) antibody [E178] in serial concentration points [3.33, 1.67, 0.83, 0.42, 0.21 nM/mL] by 2-fold dilution, next to dissociate in blank testing buffer [0.1% BSA in PBST (0.05%Tween-20)]. Calculated signals had already subtracted blank control, curve fitting using 1:1 binding model. Non-phospho Tau protein’s association and dissociation were also showed in graph. KD(M) value of Anti-Tau (phospho S396) antibody [E178] is 2.08E-11

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Immunohistochemistical detection of Tau antibody [E178] (ab32057) on formaldehyde fixed paraffin-embedded rat tongue sectionsAntigen retrieval step: Heat mediated. Buffer Used: Citric acid pH6. Permeabilization: None. Primary antibody incubated at 1/1000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG Conjugated to Biotin (1/200). A strong pattern of immunostaining which appears to be mostly localised to nerve fibres and their cell bodies (Islet of Langerhans cells are also positive). In submitted image of central area of tongue coloured arrowheads indicate features: red for nerves cut in cross-section (T/S), each brown dot representing a single axon green for what appears to me to be small nerve fibres wrapping around a partial muscle fibre black for a Ganglion containing seven positive nerve cell bodies. Surrounding these are collagen fibres (C), adipocytes (A) and skeletal/striated muscle fibres in L/S ( M-

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (ab32057)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Immunohistochemistical detection of Tau antibody [E178] (ab32057) on formaldehyde-fixed paraffin-embedded human salivary gland sections. Antigen retrieval step: Heat mediated.  Buffer Used: Citric acid pH6. Permeabilization: No.  Blocking step: 1% BSA for 10 mins @ 21°C. ab32057 incubated at 1/1000 for 2 hours @  21°C in TBS/BSA/azide. Secondary antibody:  anti rabbit IgG conjugated to Biotin (1/200). NB: An interesting pattern of positivity that seems to be supported by the Human Protein Atlas. Coloured arrowheads in the submitted image indicate features: red for positive serous glands, blue for positive intra-lobular collecting ducts, black for negative mucous glands (there is a serous demilune around this acinus), yellow for intralobular collecting ducts, green for nerve tracks in the interlobular areas, blue for positive interlobular collecting ducts. There appears to be a population of positive nuclei but this may b

  • Anti-Tau antibody [E178] (ab32057)
    Anti-Tau antibody [E178] (ab32057)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

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References (45)

Publishing research using ab32057? Please let us know so that we can cite the reference in this datasheet.

ab32057 has been referenced in 45 publications.

  • Qu Y  et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182
  • Martinez-Valbuena I  et al. Mixed pathologies in pancreatic ß cells from subjects with neurodegenerative diseases and their interaction with prion protein. Acta Neuropathol Commun 9:64 (2021). PubMed: 33832546
  • Liu Y  et al. Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition. Redox Biol 43:102002 (2021). PubMed: 33984602
  • Mao Z  et al. AMI, an Indazole Derivative, Improves Parkinson's Disease by Inhibiting Tau Phosphorylation. Front Mol Neurosci 13:165 (2020). PubMed: 33328879
  • Chen N  et al. Trilobatin Protects Against Aß25-35-Induced Hippocampal HT22 Cells Apoptosis Through Mediating ROS/p38/Caspase 3-Dependent Pathway. Front Pharmacol 11:584 (2020). PubMed: 32508629
View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Tau (phospho S396) antibody [E178]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain (Alzheimer))
Permeabilization
No
Specification
Brain (Alzheimer)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 21 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tau (phospho S396) antibody [E178]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Monocytes (Alzheimer))
Permeabilization
Yes - Triton-X 100
Specification
Monocytes (Alzheimer)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 21 2022

Western blot abreview for Anti-Tau (phospho S396) antibody [E178]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (WHOLE BRAIN LYSATE)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
WHOLE BRAIN LYSATE
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 17 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau antibody [E178]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Tongue)
Specification
Tongue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Nov 02 2010

Western blot abreview for Anti-Tau antibody [E178]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y cell line)
Loading amount
12 µg
Specification
SH-SY5Y cell line
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Víctor Campa

Verified customer

Submitted Mar 09 2010

Question

Dear technical team: Our customer performed a western blot using ab32057. He detected bands around 25 kDa not 62 kDa for ab32057 as mentioned on the datasheet. He tried to alter many steps such as primary antibody titer, incubation time, and diluent buffer, to optimize this antibody. But the results were not changed. Please comment or advice about the observed size difference. He almost used up his antibodies for optimization of conditions, so he would like to know if he can get samples.

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Abcam community

Verified customer

Asked on Apr 05 2012

Answer

Thank you for contacting Abcam regarding these antibodies. I am sorry that your customer is experiencing difficulties with these antibodies in WB. Based on the protocol information and information we have about the protein, there does not seem to be a good explanation for why you are detecting a band at the incorrect molecular weight, and importantly, at the same molecular weight. I noticed that the customer has not performed a no primary control. Is the customer sure that the secondary antibody is working well and not the cause of background? I would advise performing this important control and also consider testing another secondary antibody as well. If the secondary antibody does not appear to be the cause of the problem, I am happy to offer a replacement or credit. I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions.

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Abcam Scientific Support

Answered on Apr 05 2012

Question

Why is there such a discrepency of the KDa of the detected target in this instance?

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Abcam community

Verified customer

Asked on Jul 17 2007

Answer

Thank you for taking the time to call me yesterday. Ab30663 recognises the isoform of Tau that has a molecular weight of ~48 kDa. It is well known within the field that blotting against the non phospho form of Tau will give multiple bands (ranging from 45-65 kDa) This protein thus has multiple isoforms. The dominant isoform (even the phospho forms) will vary depending upon the species and the stage of development (which is why the other antibodies to the same target detect different sized bands). There is a good review on the dephosphorylation of Tau written by Hanger et al., that would be of use to you in this field (Hanger, DP et al, 2002 FEBS Letters, Nov 20;531(3):538-42. doi: 10.1016/s0014-5793(02)03611-6). I hope that this helps. Should you have any further questions then please do not hesitate to get back in touch with me.

Read More

Abcam Scientific Support

Answered on Jul 17 2007

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