Name: Anti-Tau (phospho S396) antibody [EPR2731]
Brand: RabMAb
SKU: ab109390
Rating: 4 (10 reviews)

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Primary - Rabbit Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Dot blot, IHC-P, IHC-Fr, WB, IP

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

Protein - Recombinant human Tau (mutated P301 S) protein (Active) (ab246005)
SDS-PAGE, Functional Studies

View more associated products

Product name

Anti-Tau (phospho S396) antibody [EPR2731]
See all Tau primary antibodies
Description

Rabbit monoclonal [EPR2731] to Tau (phospho S396)
Host species

Rabbit
Specificity

This antibody only detects Tau phosphorylated at serine 396.
Tested applications

Suitable for: Dot blot, IHC-P, IHC-Fr, WB, IPmore details
Unsuitable for: ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Tau aa 350 to the C-terminus.
Database link: P10636-8
Positive control
- WB: SH-SY5Y treated with alkaline phosphatase, Mouse brain; ICC/IF: Neuro-2a; IHC-P: human glioblastoma, human Alzheimer hippocampus; IP- Human brain lysate; IHC-Fr: Mouse and Rat cerebrum tissue.
General notes
A trial size is available to purchase for this antibody.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2731
Isotype

IgG
Research areas

Alternative Versions
- Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Immunizing Peptide (Blocking)
- Human Tau (phospho S396) peptide (ab226770)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human Tau (mutated P301 S) protein (Active) (ab246005)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Human Tau ELISA Kit (ab210972)

Our Abpromise guarantee covers the use of ab109390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Dot blot		1/1000.
IHC-P		1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr		1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
WB		1/10000 - 1/50000. Predicted molecular weight: 79 kDa.Can be blocked with Human Tau (phospho S396) peptide (ab226770). For unpurified, use 1/10000 - 1/50000.
IP		1/10 - 1/100.

Application notes

Is unsuitable for ICC/IF.

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Tissue specificity

Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Involvement in disease

Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
Sequence similarities

Contains 4 Tau/MAP repeats.
Developmental stage

Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
Domain

The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
Post-translational
modifications

Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
Cellular localization

Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Target information above from: UniProt accession P10636 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4137 Human
- Entrez Gene: 17762 Mouse
- Entrez Gene: 29477 Rat
- Omim: 157140 Human
- SwissProt: P10636 Human
- SwissProt: P10637 Mouse
- SwissProt: P19332 Rat
- Unigene: 101174 Human
- Unigene: 1287 Mouse
- Unigene: 2455 Rat
see all
Form

There are 9 isoforms produced by alternative splicing.
Alternative names
- AI413597 antibody
- AW045860 antibody
- DDPAC antibody
- FLJ31424 antibody
- FTDP 17 antibody
- G protein beta1/gamma2 subunit interacting factor 1 antibody
- MAPT antibody
- MAPTL antibody
- MGC134287 antibody
- MGC138549 antibody
- MGC156663 antibody
- Microtubule associated protein tau antibody
- Microtubule associated protein tau isoform 4 antibody
- Microtubule-associated protein tau antibody
- MSTD antibody
- Mtapt antibody
- MTBT1 antibody
- MTBT2 antibody
- Neurofibrillary tangle protein antibody
- Paired helical filament tau antibody
- Paired helical filament-tau antibody
- PHF tau antibody
- PHF-tau antibody
- PPND antibody
- PPP1R103 antibody
- Protein phosphatase 1, regulatory subunit 103 antibody
- pTau antibody
- RNPTAU antibody
- TAU antibody
- TAU_HUMAN antibody
- Tauopathy and respiratory failure antibody
- Tauopathy and respiratory failure, included antibody
see all

Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

All lanes : Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/20000 dilution (purified)

Lane 1 : Untreated SH-SY5Y
Lane 2 : SH-SY5Y treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat ant-rabbit (H+L) at 1/1000 dilution

Predicted band size: 79 kDa
Observed band size: 50-70 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

Immunohistochemistry (Frozen sections) analysis of rat cerebrum tissue sections labeling Tau with Purified ab109390 at 1/100 (1.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

Immunohistochemical staining of paraffin embedded human glioblastoma with purified ab109390 at a dilution of 1/4000. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

ab109390 at 1/20 immunoprecipitating Tau (phospho S396) in Human brain lysate.

Lane 1 (input): Human brain lysate (10µg)

Lane 2 (+): ab109390 + Human brain lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109390 in Human brain lysate.

For western blotting, ab109390 at 1/1000 dilution followed by VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Diluting / Blocking buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with Purified ab109390 at 1/100 (1.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)Image courtesy of Carl Hobbs, Kings College London, U.K.

IHC image of Tau (phospho S396) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified ab109390 at 1/1000 dilution for 2 hoursat 21ºC. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.
See Abreview
Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

All lanes : Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/20000 dilution

Lane 1 : Human brain whole cell lysates.
Lane 2 : Human brain whole cell lysates. The membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 79 kDa

Exposure time: 1 minute

Diluting and blocking buffer: 5% NFDM/TBST
Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/5000 dilution (purified) + Mouse brain at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 79 kDa
Observed band size: 50-70 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Dot Blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

Dot blot analysis of Tau (phospho S396) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S396) with ab109390 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

All lanes : Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/10000 dilution (unpurified)

Lane 1 : SH-SY5Y cell lysates, untreated
Lane 2 : SH-SY5Y cell lysates, treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 79 kDa

Western blot protocols
Immunohistochemistry protocols

This product has been referenced in:

Huang R et al. Increased Ratio of Global O-GlcNAcylation to Tau Phosphorylation at Thr212 Site Is Associated With Better Memory Function in Patients With Type 2 Diabetes. Front Physiol 10:110 (2019). Read more (PubMed: 30837891) »
Chen X et al. Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aß1-42-Induced Alzheimer's Disease in Mice by Activating ß-Catenin Signaling. Biomed Res Int 2019:1431760 (2019). Read more (PubMed: 30949496) »

See all 44 Publications for this product

Publishing research using ab109390? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 6

Permeabilization

Specification

Brain

Fixative

Formaldehyde

Zohar Gavish

Verified customer

Submitted Mar 15 2019

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (brain)

Permeabilization

Specification

brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Fixative

Paraformaldehyde

Mr. Tamas Bellak

Verified customer

Submitted Aug 24 2018

Application

Immunohistochemistry free floating

Sample

Rat Tissue sections (Brain)

Specification

Brain

Abcam user community

Verified customer

Submitted Mar 29 2018

Application

Western blot

Sample

Pig Tissue lysate - whole (brain)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Treatment

traumatic brain injury, 30days post injury

Specification

brain

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 27 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Specification

Brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Fixative

Formaldehyde

Dr. Tom Duncan

Verified customer

Submitted Oct 18 2017

Application

ELISA

Sample

Mouse Tissue sections (Cortex protein lysate)

Specification

Cortex protein lysate

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 18°C

Type

Sandwich (Detection)

Dr. Eitan Okun

Verified customer

Submitted Aug 23 2016

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Brain)

Permeabilization

Yes - Buffer solutions for heat-induced epitope retrieval

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: rt°C

Fixative

Paraformaldehyde

Dr. Eitan Okun

Verified customer

Submitted Apr 18 2016

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - whole cell (human stem cell derived neurons (healthy/diseased))

Specification

human stem cell derived neurons (healthy/diseased)

Treatment

quinolinic acid 72h (and non treated)

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Mar 26 2014

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

(agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Sample

Human Tissue sections (Brain)

Specification

Brain

Permeabilization

Fixative

Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 12 2013

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Sample

Human Tissue sections (Tau/APP transgenic mouse brain expressing human Ta)

Specification

Tau/APP transgenic mouse brain expressing human Ta

Permeabilization

Fixative

Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 12 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Immunizing Peptide (Blocking)

Isotype control

Recombinant Protein

Related Products

Applications

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Developmental stage

Domain

Post-translationalmodifications

Cellular localization

Database links

Form

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Immunohistochemistry (Frozen sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Immunohistochemistry free floating abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Western blot abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

ELISA abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Immunohistochemistry (Frozen sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Western blot abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Post-translational
modifications