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    tau-phospho-s396-antibody-epr2731-ab109390.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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RecombinantRabMAb

Recombinant Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

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Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Dot Blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2731] to Tau (phospho S396)
  • Suitable for: Dot blot, IHC-Fr, IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-Tau (phospho S396) antibody [EPR2731]
    See all Tau primary antibodies
  • Description

    Rabbit monoclonal [EPR2731] to Tau (phospho S396)
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: Dot blot, IHC-Fr, IHC-P, WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab226770)

  • Positive control

    • WB: SH-SY5Y treated with alkaline phosphatase, Human and Mouse brain tissue lysate; IHC-P: human glioblastoma, human Alzheimer hippocampus, human, mouse and rat colon; IP- Human brain lysate; IHC-Fr: Mouse and Rat cerebrum tissue, Hu Alzheimer brain.
  • General notes

    Tau is a protein associated with several disease states, known collectively as tauopathies. The most well-known of these is Alzheimer’s disease (AD), were tau exhibiting excessive phosphorylation, aggregating to form neurofibrillary tangles. The epitope defined by phosphorylation of S396 in tau is strongly implicated in AD-associated tau pathology, providing a valuable target for the development of therapeutic antibodies to capture tau and prevent spreading of tau pathology. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2731
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Development
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunizing Peptide (Blocking)

    • Human Tau (phospho S396) peptide (ab226770)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/1000.
IHC-Fr (2)
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

IHC-P (5)
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

WB (3)
1/10000 - 1/50000. Predicted molecular weight: 79 kDa.Can be blocked with Human Tau (phospho S396) peptide (ab226770).

For unpurified, use 1/10000 - 1/50000.

IP
1/10 - 1/100.
Notes
Dot blot
1/1000.
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

WB
1/10000 - 1/50000. Predicted molecular weight: 79 kDa.Can be blocked with Human Tau (phospho S396) peptide (ab226770).

For unpurified, use 1/10000 - 1/50000.

IP
1/10 - 1/100.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • Tissue specificity

    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
  • Involvement in disease

    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
  • Sequence similarities

    Contains 4 Tau/MAP repeats.
  • Developmental stage

    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
  • Domain

    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
  • Post-translational
    modifications

    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
  • Cellular localization

    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Target information above from: UniProt accession P10636 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4137 Human
    • Entrez Gene: 17762 Mouse
    • Entrez Gene: 29477 Rat
    • Omim: 157140 Human
    • SwissProt: P10636 Human
    • SwissProt: P10637 Mouse
    • SwissProt: P19332 Rat
    • Unigene: 101174 Human
    • Unigene: 1287 Mouse
    • Unigene: 2455 Rat
    see all
  • Form

    There are 9 isoforms produced by alternative splicing.
  • Alternative names

    • AI413597 antibody
    • AW045860 antibody
    • DDPAC antibody
    • FLJ31424 antibody
    • FTDP 17 antibody
    • G protein beta1/gamma2 subunit interacting factor 1 antibody
    • MAPT antibody
    • MAPTL antibody
    • MGC134287 antibody
    • MGC138549 antibody
    • MGC156663 antibody
    • Microtubule associated protein tau antibody
    • Microtubule associated protein tau isoform 4 antibody
    • Microtubule-associated protein tau antibody
    • MSTD antibody
    • Mtapt antibody
    • MTBT1 antibody
    • MTBT2 antibody
    • Neurofibrillary tangle protein antibody
    • Paired helical filament tau antibody
    • Paired helical filament-tau antibody
    • PHF tau antibody
    • PHF-tau antibody
    • PPND antibody
    • PPP1R103 antibody
    • Protein phosphatase 1, regulatory subunit 103 antibody
    • pTau antibody
    • RNPTAU antibody
    • TAU antibody
    • TAU_HUMAN antibody
    • Tauopathy and respiratory failure antibody
    • Tauopathy and respiratory failure, included antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

    Immunohistochemistry analysis of frozen mouse cerebrum tissue sections labeling Tau (phospho S396) with ab109390 at 1/100 (1 μg/mL). ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Cytoplasmic staining on mouse cerebrum, the signal decreased after phosphatase treatment at 37℃ for 2h.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
  • Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    All lanes : Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/1000 dilution

    Lane 1 : Human brain lysates
    Lane 2 : Human brain lysates and the membrane was incubated with alkaline phosphatase
    Lane 3 : Human brain lysates and the membrane was incubated with lambda phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50-79 kDa why is the actual band size different from the predicted?


    Exposure time: 100 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733.

  • Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    All lanes : Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/1000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse brain lysates and the membrane was incubated with alkaline phosphatase
    Lane 3 : Mouse brain lysates and the membrane was incubated with lambda phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50-79 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Diluting/Diluting buffer and concentration 5% NFDM/TBST

    Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733..

  • Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    All lanes : Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/20000 dilution (purified)

    Lane 1 : Untreated SH-SY5Y
    Lane 2 : SH-SY5Y treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat ant-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

    IHC image of Tau (phospho S396) staining in a section of frozen normal human Alzheimer brain performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab109390, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

    Immunohistochemical staining of paraffin embedded human glioblastoma with purified ab109390 at a dilution of 1/4000. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

    ab109390 at 1/20 immunoprecipitating Tau (phospho S396) in Human brain lysate.

    Lane 1 (input): Human brain lysate (10µg)

    Lane 2 (+): ab109390 + Human brain lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109390 in Human brain lysate.

    For western blotting, ab109390  at 1/1000 dilution followed by VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Diluting / Blocking buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

    Immunohistochemistry analysis of paraffin-embedded human colon tissue sections labelling Tau (phospho S396) with ab109390 at 1/4000 dilution (0.026 μg/mL). The section was incubated with ab109390 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
    Positive staining on ganglions of human colon without alkaline phosphatase treatment (image A); No signal was detected when tissues were treated with alkaline phosphatase (image B).The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)Image courtesy of Carl Hobbs, Kings College London, U.K.

    IHC image of Tau (phospho S396) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified ab109390 at 1/1000 dilution for 2 hoursat 21ºC. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.

    See Abreview

  • Dot Blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Dot Blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

    Dot blot analysis of Tau (phospho S396) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S396) with ab109390 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
    Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (82)

Publishing research using ab109390? Please let us know so that we can cite the reference in this datasheet.

ab109390 has been referenced in 82 publications.

  • Heyburn L  et al. Repeated Low-Level Blast Acutely Alters Brain Cytokines, Neurovascular Proteins, Mechanotransduction, and Neurodegenerative Markers in a Rat Model. Front Cell Neurosci 15:636707 (2021). PubMed: 33679327
  • Shi YH  et al. Neuroprotective Effect of Astragaloside IV on Cerebral Ischemia/Reperfusion Injury Rats Through Sirt1/Mapt Pathway. Front Pharmacol 12:639898 (2021). PubMed: 33841157
  • Zhang YY  et al. Inhibition of interleukin-1ß plays a protective role in Alzheimer's disease by promoting microRNA-9-5p and downregulating targeting protein for xenopus kinesin-like protein 2. Int Immunopharmacol 97:107578 (2021). PubMed: 33892301
  • Liu Y  et al. Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition. Redox Biol 43:102002 (2021). PubMed: 33984602
  • Abey A  et al. Distribution of tau hyperphosphorylation in canine dementia resembles early Alzheimer's disease and other tauopathies. Brain Pathol 31:144-162 (2021). PubMed: 32810333
View all Publications for this product

Customer reviews and Q&As

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1-10 of 13 Abreviews

Western blot abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (WHOLE BRAIN LYSATE)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
WHOLE BRAIN LYSATE
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 17 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (brain organoid)
Permeabilization
Yes - 0.5%tritonX-100
Specification
brain organoid
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 22 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Ethanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Lindsay Meyerdirk

Verified customer

Submitted Apr 13 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6
Permeabilization
No
Specification
Brain
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Zohar Gavish

Verified customer

Submitted Mar 15 2019

Immunohistochemistry (Frozen sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Tamas Bellak

Verified customer

Submitted Aug 24 2018

Immunohistochemistry free floating abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 29 2018

Western blot abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
traumatic brain injury, 30days post injury
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 27 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tom Duncan

Verified customer

Submitted Oct 18 2017

ELISA abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Good
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Mouse Tissue sections (Cortex protein lysate)
Specification
Cortex protein lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 18°C
Type
Sandwich (Detection)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Eitan Okun

Verified customer

Submitted Aug 23 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Tau (phospho S396) antibody [EPR2731]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Buffer solutions for heat-induced epitope retrieval
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: rt°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Eitan Okun

Verified customer

Submitted Apr 18 2016

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