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  1. Link

    tau-phospho-s404-antibody-epr2605-ab92676.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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RecombinantRabMAb

Recombinant Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

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Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Dot Blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
  • Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2605] to Tau (phospho S404)
  • Suitable for: IP, WB, IHC-P, ICC, IHC-Fr, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Tau (phospho S404) antibody [EPR2605]
    See all Tau primary antibodies
  • Description

    Rabbit monoclonal [EPR2605] to Tau (phospho S404)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC, IHC-Fr, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Mouse primary cortical cultures; WB: Human, mouse and rat brain lysate; IHC: Mouse and rat cerebrum and Human glioma tissue; IP: Human brain lysate, IHC-Fr: Mouse cerebrum tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.01% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2605
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Development
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/20.
WB (3)
1/500 - 1/2000. Predicted molecular weight: 79 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
1/50.
IHC-Fr
1/50.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Dot blot
Use at an assay dependent concentration.
Notes
IP
1/20.
WB
1/500 - 1/2000. Predicted molecular weight: 79 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
1/50.
IHC-Fr
1/50.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Dot blot
Use at an assay dependent concentration.

Target

  • Function

    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • Tissue specificity

    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
  • Involvement in disease

    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
  • Sequence similarities

    Contains 4 Tau/MAP repeats.
  • Developmental stage

    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
  • Domain

    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
  • Post-translational
    modifications

    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
  • Cellular localization

    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Target information above from: UniProt accession P10636 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4137 Human
    • Entrez Gene: 17762 Mouse
    • Entrez Gene: 29477 Rat
    • Omim: 157140 Human
    • SwissProt: P10636 Human
    • SwissProt: P10637 Mouse
    • SwissProt: P19332 Rat
    • Unigene: 101174 Human
    • Unigene: 1287 Mouse
    • Unigene: 2455 Rat
    see all
  • Form

    There are 9 isoforms produced by alternative splicing.
  • Alternative names

    • AI413597 antibody
    • AW045860 antibody
    • DDPAC antibody
    • FLJ31424 antibody
    • FTDP 17 antibody
    • G protein beta1/gamma2 subunit interacting factor 1 antibody
    • MAPT antibody
    • MAPTL antibody
    • MGC134287 antibody
    • MGC138549 antibody
    • MGC156663 antibody
    • Microtubule associated protein tau antibody
    • Microtubule associated protein tau isoform 4 antibody
    • Microtubule-associated protein tau antibody
    • MSTD antibody
    • Mtapt antibody
    • MTBT1 antibody
    • MTBT2 antibody
    • Neurofibrillary tangle protein antibody
    • Paired helical filament tau antibody
    • Paired helical filament-tau antibody
    • PHF tau antibody
    • PHF-tau antibody
    • PPND antibody
    • PPP1R103 antibody
    • Protein phosphatase 1, regulatory subunit 103 antibody
    • pTau antibody
    • RNPTAU antibody
    • TAU antibody
    • TAU_HUMAN antibody
    • Tauopathy and respiratory failure antibody
    • Tauopathy and respiratory failure, included antibody
    see all

Images

  • Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

    Purified ab92676 at 1/20 dilution (0.5µg) immunoprecipitating Tau in Mouse brain lysate.
    Lane 1 (input): Mouse brain lysate 10µg
    Lane 2 (+): ab92676 + Mouse brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92676 in Mouse brain lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 50-70 kDa

  • Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

    Purified ab92676 at 1/20 dilution (0.5µg) immunoprecipitating Tau in Human brain lysate.
    Lane 1 (input): Human brain lysate 10µg
    Lane 2 (+): ab92676 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92676 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 50-70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Tau with purified ab92676 at 1/200 dilution (0.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling Tau with purified ab92676 at 1/200 dilution (0.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Tau with purified ab92676 at 1/200 dilution (0.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    All lanes : Anti-Tau (phospho S404) antibody [EPR2605] (ab92676) at 1/1000 dilution (Purified)

    Lane 1 : Rat brain lysate
    Lane 2 : Rat brain lysate, the menbrane treated with alkaline phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 79 kDa

  • Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    All lanes : Anti-Tau (phospho S404) antibody [EPR2605] (ab92676) at 1/1000 dilution (Purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse brain lysate, the menbrane treated with alkaline phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 79 kDa

  • Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    All lanes : Anti-Tau (phospho S404) antibody [EPR2605] (ab92676) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate, the menbrane treated with alkaline phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 79 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

    Immunocytochemistry/ Immunofluorescence analysis of Embryonic mouse primary neural cells labeling Tau with purified ab92676 at 1:100 dilution (1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. 

    Confocal image showing positive staining in neuron.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with Purified ab92676 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)This image is courtesy of an anonymous Abreview.

    Mouse primary cortical cultures stained for Tau (phospho S404) (green) using ab92676 at 1/100 dilution in ICC/IF, followed by Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488®.

    See Abreview

  • Dot Blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Dot Blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

    Dot blot analysis of Tau (pS404) phosopho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (phospho S404) with ab92676 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)
    Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (17)

Publishing research using ab92676? Please let us know so that we can cite the reference in this datasheet.

ab92676 has been referenced in 17 publications.

  • Zuliani I  et al. The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome. Neurotherapeutics 18:340-363 (2021). PubMed: 33258073
  • Guo Y  et al. Ginsenoside Rg1 improves cognitive capability and affects the microbiota of large intestine of tree shrew model for Alzheimer's disease. Mol Med Rep 23:N/A (2021). PubMed: 33649817
  • Qu Y  et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182
  • Li D & Cho YK High specificity of widely used phospho-tau antibodies validated using a quantitative whole-cell based assay. J Neurochem 152:122-135 (2020). PubMed: 31325178
  • Díaz-Zúñiga J  et al. Alzheimer's Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent. Front Immunol 11:588036 (2020). PubMed: 33240277
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (WHOLE BRAIN LYSATE)
Gel Running Conditions
Reduced Denaturing (3-8%)
Loading amount
40 µg
Specification
WHOLE BRAIN LYSATE
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 20 2022

Western blot abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Basal Forebrain Cholinergic Neurons)
Gel Running Conditions
Reduced Denaturing (8% Acrylamide)
Loading amount
5 µg
Specification
Basal Forebrain Cholinergic Neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

DR. Martin Engel

Verified customer

Submitted May 23 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (primary cortical cultures)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
primary cortical cultures
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 13 2016

Western blot abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - other (human stem cell derived neurons (healthy/diseased))
Specification
human stem cell derived neurons (healthy/diseased)
Treatment
quinolinic acid 72h (and non treated)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Read More

Abcam user community

Verified customer

Submitted Mar 26 2014

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