Name: Anti-Tau (phospho S404) antibody [EPR2605]
Brand: RabMAb
SKU: ab92676
Rating: 5 (4 reviews)

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Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2605] to Tau (phospho S404)
Suitable for: IP, WB, IHC-P, ICC, IHC-Fr, Dot blot
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Tau (phospho S404) antibody [EPR2605]
See all Tau primary antibodies
Description

Rabbit monoclonal [EPR2605] to Tau (phospho S404)
Host species

Rabbit
Tested applications

Suitable for: IP, WB, IHC-P, ICC, IHC-Fr, Dot blotmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: Mouse primary cortical cultures; WB: Human, mouse and rat brain lysate; IHC: Mouse and rat cerebrum and Human glioma tissue; IP: Human brain lysate, IHC-Fr: Mouse cerebrum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.01% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2605
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Diseases
- Neuroscience
- Processes

Alternative Versions
- Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		1/20.
WB	(3)	1/500 - 1/2000. Predicted molecular weight: 79 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC		1/50.
IHC-Fr		1/50. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Dot blot		Use at an assay dependent concentration.

Notes
IP 1/20.
WB 1/500 - 1/2000. Predicted molecular weight: 79 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50.
IHC-Fr 1/50. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Dot blot Use at an assay dependent concentration.

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Tissue specificity

Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Involvement in disease

Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
Sequence similarities

Contains 4 Tau/MAP repeats.
Developmental stage

Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
Domain

The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
Post-translational
modifications

Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
Cellular localization

Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Target information above from: UniProt accession P10636 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4137 Human
- Entrez Gene: 17762 Mouse
- Entrez Gene: 29477 Rat
- Omim: 157140 Human
- SwissProt: P10636 Human
- SwissProt: P10637 Mouse
- SwissProt: P19332 Rat
- Unigene: 101174 Human
- Unigene: 1287 Mouse
- Unigene: 2455 Rat
see all
Form

There are 9 isoforms produced by alternative splicing.
Alternative names
- AI413597 antibody
- AW045860 antibody
- DDPAC antibody
- FLJ31424 antibody
- FTDP 17 antibody
- G protein beta1/gamma2 subunit interacting factor 1 antibody
- MAPT antibody
- MAPTL antibody
- MGC134287 antibody
- MGC138549 antibody
- MGC156663 antibody
- Microtubule associated protein tau antibody
- Microtubule associated protein tau isoform 4 antibody
- Microtubule-associated protein tau antibody
- MSTD antibody
- Mtapt antibody
- MTBT1 antibody
- MTBT2 antibody
- Neurofibrillary tangle protein antibody
- Paired helical filament tau antibody
- Paired helical filament-tau antibody
- PHF tau antibody
- PHF-tau antibody
- PPND antibody
- PPP1R103 antibody
- Protein phosphatase 1, regulatory subunit 103 antibody
- pTau antibody
- RNPTAU antibody
- TAU antibody
- TAU_HUMAN antibody
- Tauopathy and respiratory failure antibody
- Tauopathy and respiratory failure, included antibody
see all

Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Purified ab92676 at 1/20 dilution (0.5µg) immunoprecipitating Tau in Mouse brain lysate.
Lane 1 (input): Mouse brain lysate 10µg
Lane 2 (+): ab92676 + Mouse brain lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92676 in Mouse brain lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 50-70 kDa
Immunoprecipitation - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Purified ab92676 at 1/20 dilution (0.5µg) immunoprecipitating Tau in Human brain lysate.
Lane 1 (input): Human brain lysate 10µg
Lane 2 (+): ab92676 + Human brain lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92676 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 50-70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Tau with purified ab92676 at 1/200 dilution (0.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling Tau with purified ab92676 at 1/200 dilution (0.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Tau with purified ab92676 at 1/200 dilution (0.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

All lanes : Anti-Tau (phospho S404) antibody [EPR2605] (ab92676) at 1/1000 dilution (Purified)

Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate, the menbrane treated with alkaline phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 79 kDa
Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

All lanes : Anti-Tau (phospho S404) antibody [EPR2605] (ab92676) at 1/1000 dilution (Purified)

Lane 1 : Mouse brain lysate
Lane 2 : Mouse brain lysate, the menbrane treated with alkaline phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 79 kDa
Western blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

All lanes : Anti-Tau (phospho S404) antibody [EPR2605] (ab92676) at 1/1000 dilution (Purified)

Lane 1 : Human brain lysate
Lane 2 : Human brain lysate, the menbrane treated with alkaline phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 79 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Immunocytochemistry/ Immunofluorescence analysis of Embryonic mouse primary neural cells labeling Tau with purified ab92676 at 1:100 dilution (1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor^© 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1:200 (2.5 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain.

Confocal image showing positive staining in neuron.
Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with Purified ab92676 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)This image is courtesy of an anonymous Abreview.

Mouse primary cortical cultures stained for Tau (phospho S404) (green) using ab92676 at 1/100 dilution in ICC/IF, followed by Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488^®.
See Abreview
Dot Blot - Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Dot blot analysis of Tau (pS404) phosopho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (phospho S404) with ab92676 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-Tau (phospho S404) antibody [EPR2605] (ab92676)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab92676? Please let us know so that we can cite the reference in this datasheet.

ab92676 has been referenced in 17 publications.

Zuliani I et al. The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome. Neurotherapeutics 18:340-363 (2021). PubMed: 33258073
Guo Y et al. Ginsenoside Rg1 improves cognitive capability and affects the microbiota of large intestine of tree shrew model for Alzheimer's disease. Mol Med Rep 23:N/A (2021). PubMed: 33649817
Qu Y et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182
Li D & Cho YK High specificity of widely used phospho-tau antibodies validated using a quantitative whole-cell based assay. J Neurochem 152:122-135 (2020). PubMed: 31325178
Díaz-Zúñiga J et al. Alzheimer's Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent. Front Immunol 11:588036 (2020). PubMed: 33240277

View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Human Tissue lysate - whole (WHOLE BRAIN LYSATE)

Gel Running Conditions

Reduced Denaturing (3-8%)

Loading amount

40 µg

Specification

WHOLE BRAIN LYSATE

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 20 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (Basal Forebrain Cholinergic Neurons)

Gel Running Conditions

Reduced Denaturing (8% Acrylamide)

Loading amount

5 µg

Specification

Basal Forebrain Cholinergic Neurons

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

DR. Martin Engel

Verified customer

Submitted May 23 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (primary cortical cultures)

Permeabilization

Yes - 0.25% Triton X-100 in PBS

Specification

primary cortical cultures

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 13 2016

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - other (human stem cell derived neurons (healthy/diseased))

Specification

human stem cell derived neurons (healthy/diseased)

Treatment

quinolinic acid 72h (and non treated)

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Mar 26 2014

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Developmental stage

Domain

Post-translationalmodifications

Cellular localization

Database links

Form

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (17)

Customer reviews and Q&As

Western blot abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Western blot abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Western blot abreview for Anti-Tau (phospho S404) antibody [EPR2605]

Post-translational
modifications