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    tau-phospho-t205-antibody-ab4841.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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Anti-Tau (phospho T205) antibody (ab4841)

  • Datasheet
Submit a review Q&A (1)References (9)

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Western blot - Anti-Tau (phospho T205) antibody (ab4841)
  • Flow Cytometry - Anti-Tau (phospho T205) antibody (ab4841)

Key features and details

  • Rabbit polyclonal to Tau (phospho T205)
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG

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Primary
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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human Tau (mutated P301S) protein (Active) (ab246005)

View more associated products

Overview

  • Product name

    Anti-Tau (phospho T205) antibody
    See all Tau primary antibodies
  • Description

    Rabbit polyclonal to Tau (phospho T205)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (Human) derived from the region of human tau that contains threonine 205.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at threonine 205.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Tau (mutated P301S) protein (Active) (ab246005)
  • Related Products

    • Human Tau ELISA Kit (ab210972)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
All applications
Rat
Application Abreviews Notes
Flow Cyt
WB
Notes
  • Application notes
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 62 kDa. Can be blocked with Tau peptide - phospho T205 (phospho and non-phospho pair).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
    • Tissue specificity

      Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
    • Involvement in disease

      Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
      Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
      Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
      Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
      Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
    • Sequence similarities

      Contains 4 Tau/MAP repeats.
    • Developmental stage

      Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
    • Domain

      The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
    • Post-translational
      modifications

      Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
      Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
      Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
    • Cellular localization

      Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
    • Target information above from: UniProt accession P10636 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4137 Human
      • Entrez Gene: 29477 Rat
      • Omim: 157140 Human
      • SwissProt: P10636 Human
      • SwissProt: P19332 Rat
      • Unigene: 101174 Human
      • Unigene: 2455 Rat
      • Form

        There are 9 isoforms produced by alternative splicing.
      • Alternative names

        • AI413597 antibody
        • AW045860 antibody
        • DDPAC antibody
        • FLJ31424 antibody
        • FTDP 17 antibody
        • G protein beta1/gamma2 subunit interacting factor 1 antibody
        • MAPT antibody
        • MAPTL antibody
        • MGC134287 antibody
        • MGC138549 antibody
        • MGC156663 antibody
        • Microtubule associated protein tau antibody
        • Microtubule associated protein tau isoform 4 antibody
        • Microtubule-associated protein tau antibody
        • MSTD antibody
        • Mtapt antibody
        • MTBT1 antibody
        • MTBT2 antibody
        • Neurofibrillary tangle protein antibody
        • Paired helical filament tau antibody
        • Paired helical filament-tau antibody
        • PHF tau antibody
        • PHF-tau antibody
        • PPND antibody
        • PPP1R103 antibody
        • Protein phosphatase 1, regulatory subunit 103 antibody
        • pTau antibody
        • RNPTAU antibody
        • TAU antibody
        • TAU_HUMAN antibody
        • Tauopathy and respiratory failure antibody
        • Tauopathy and respiratory failure, included antibody
        see all

      Images

      • Western blot - Anti-Tau (phospho T205) antibody (ab4841)
        Western blot - Anti-Tau (phospho T205) antibody (ab4841)

        WB using Ab4841. The peptide corresponding to tau [pT205] blocks the antibody signal, thereby demonstrating the specificity of the antibody. 

      • Flow Cytometry - Anti-Tau (phospho T205) antibody (ab4841)
        Flow Cytometry - Anti-Tau (phospho T205) antibody (ab4841)

        Flow cytometric analysis of 70% ethanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling Tau (phospho T205) with ab4841 at 5 μg/million cells (red) compared with a rabbit Isotype control details (pink),an unlabelled control (purple) and a secondary only control (green). Alexa Fluor® 488 Goat Anti-Rabbit, at 1/400 dilution was used as the secondary antibody.

      Protocols

      • Peptide Competition Experiment
      • Western blotting protocol

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (9)

      Publishing research using ab4841? Please let us know so that we can cite the reference in this datasheet.

      ab4841 has been referenced in 9 publications.

      • Bathini P  et al. Progressive signaling changes in the olfactory nerve of patients with Alzheimer's disease. Neurobiol Aging 76:80-95 (2019). PubMed: 30708185
      • Moosecker S  et al. Activated PPAR? Abrogates Misprocessing of Amyloid Precursor Protein, Tau Missorting and Synaptotoxicity. Front Cell Neurosci 13:239 (2019). PubMed: 31263400
      • Liu Z  et al. Amyloid ß and tau are involved in sleep disorder in Alzheimer's disease by orexin A and adenosine A(1) receptor. Int J Mol Med 43:435-442 (2019). PubMed: 30365112
      • Tong M  et al. T3D-959: A Multi-Faceted Disease Remedial Drug Candidate for the Treatment of Alzheimer's Disease. J Alzheimers Dis 51:123-38 (2016). PubMed: 26836193
      • Brai E  et al. Notch1 hallmarks fibrillary depositions in sporadic Alzheimer's disease. Acta Neuropathol Commun 4:64 (2016). PubMed: 27364742
      • Hamm M  et al. Physiologically relevant factors influence tau phosphorylation by leucine-rich repeat kinase 2. J Neurosci Res 93:1567-80 (2015). PubMed: 26123245
      • Krstic D  et al. Systemic immune challenges trigger and drive Alzheimer-like neuropathology in mice. J Neuroinflammation 9:151 (2012). WB, IHC-FrFl ; Mouse . PubMed: 22747753
      • Kocherhans S  et al. Reduced Reelin expression accelerates amyloid-beta plaque formation and tau pathology in transgenic Alzheimer's disease mice. J Neurosci 30:9228-40 (2010). IHC-FoFr . PubMed: 20610758
      • Nathan C  et al. Protection from Alzheimer's-like disease in the mouse by genetic ablation of inducible nitric oxide synthase. J Exp Med 202:1163-9 (2005). PubMed: 16260491

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I am currently working on an Alzheimer's project and would like to use one of your Tau antibodies in order to visualize abnormal tau in transgenic mice. Do you know if ab4841 is an appropriate antibody or if you have any others that might be better for the job? Thanks

      Read More

      Abcam community

      Verified customer

      Asked on Apr 24 2006

      Answer

      Thank you for your inquiry. ab4841 is a good antibody to use if you are specifically interested in identifying tau that is phosphorylated at amino acid residue threonine 205. The best advice I can give you is to perform a literature search and then search our website using the appropriate filters to find an antibody that reacts with the species in which you are interested. It is very difficult to tell you that you should use this particular antibody without know more about your studies. Of note, Dr. Lester Binder's lab has many tau antibodies. Upon reading his papers, it is apparent that whichever tau antibody is used can really influence what is actually being examined. Overall, our antibodies will work as described on the datasheet, but unfortunately, recommending one specific antibody without knowing your specific research goal is not prudent.

      Read More

      Abcam Scientific Support

      Answered on Apr 26 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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