Overview

  • Product name
    Anti-Taxol antibody
  • Description
    Rabbit polyclonal to Taxol
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Taxol conjugation tg (Thyroglobulin bovine).

Properties

Applications

Our Abpromise guarantee covers the use of ab26953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: 1/10000.
    Antigen is coated on EIA strips at 0.4 µg per well. Antiserum is diluted in series from 1/10 to 1/10,000 and added in separate wells.
    Incubate the antibody for 0.5 hr. Wash off unbound antibodies and add anti-rabbit IgG-HRP conjugate. Wash the plates and add substrate to develop color for 5 min. Read absorbance at 405 nm. The intensity of the colour is directly proportional to the amount of antigen present.
    A positive is when the sample signal is double that of the control or pre-immune serum.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Taxol (paclitaxel) is particularly effective as an anticancer agent because tubulin is not its only target. It also binds to a protein called Bcl2, which normally blocks the process of apoptosis, or cell death. Taxol therefore plays a dual role in destroying dividing cells,firstly by stabilising assembled microtubules and thereby halting mitosis, and secondly by inhibiting Bcl2 and allowing apoptosis to proceed.
    • Alternative names
      • Paclitaxel antibody

    References

    ab26953 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Application
    ELISA
    Sample
    Rat Serum (oral)
    Specification
    oral
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Type
    Indirect

    Abcam user community

    Verified customer

    Submitted Dec 14 2015

    Answer

    Thank you for contacting us.

    To our knowledge, the anti-Taxol ab26953 has not been tested in immunocytochemistry. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing ab26953 in immunocytochemistry.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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    Answer

    Thank you for contacting us.

    The titre of this antibody as observed in indirect ELISA is more than 10,000.

    The below table show absorbance at 405nm using 0.4ug of antigen. HRP-conjugated anti rabbit ab was used.

    Ab= dilution / Control antiserum / Antiserum ab26953

    1:10,000 / 6.769 / 11.442

    1:1,000 / 6.402 / 15.622

    1:100 /10.650 / 22.400

    1:10 /5.216 / 20.468

    Titer >10K

    Antibody is positive at >2 folds of ABS of control/Pre-Immune serum.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you for your enquiry.

    I would appreciate if you could confirm you are referring toTaxol antibody product code ab26953?

    I am sorry to confirm that as far as we are aware, ab26953 has never been tested in ICC. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    If you would like to test ab26953 in ICC, please do not hesitate tocontactme againprior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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    Answer

    Thank you for your inquiry and your patience with this reply.
    I am sorry to report that the lab hasn't tested to verify the position of the conjugate on taxol. They do not have this information.
    I regret that this information has not been very helpful, but please contact us with any other questions.

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    Question
    Answer

    Thank you for contacting us.

    The link to our protocol for indirect ELISA is as follows:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11389

    Another useful link to our practical guide for ELISA is:

    https://www.abcam.com/index.html?pageconfig=resource&rid=14665

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question
    Answer

    Thank you for your enquiry. Unfortunately, we currently do not have any direct references regarding the use of this product. All of our references come from loyal customers like you, who have used our products, published their findings in journals and later inform us of their publications. However, I managed to locate some publications on the subject that might contain useful information. Due to my limited access to journals, I was not able to check to see if they are relevant to what you are looking for. I sincerely hope that these journals will be able to provide some help. 1) Marone M, D'Andrilli G, Das N, Ferlini C, Chatterjee S, Scambia G. Quercetin abrogates taxol mediated signaling by inhibiting multiple kinases. Exp Cell Res 2001; 270(1):1-12 2) Amos LA, Lowe J. How Taxol stabilises microtubule structure. Chem Bio 1999;6(3):R65-9 Please rest assure that if the product does not perform in species and application as stated on the datasheet, and the problem (not protocol related) has been reported to us within 120 days of purchase, we will be more than happy to offer you a free replacement or refund. Should you require any other assistance, please do not hesitate to contact me.

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    Answer

    Thank you for your enquiry. Please rest assure that this product (ab26953) is an antibody, raised by immunizing rabbits with the Taxol conjugation tg. It is not the Taxol compound itself and would therefore not cause any health problems. This product is intended for research use only and not intended for diagnostic or therapeutic purposes. Should you have any doubts, please consult your nearest physician. I hope this information will settle your worries. Should you require any further information, please do not hesitate to contact me. Good luck in your research.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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