The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antigen is coated on EIA strips at 0.4 µg per well. Antiserum is diluted in series from 1/10 to 1/10,000 and added in separate wells.
Incubate the antibody for 0.5 hr. Wash off unbound antibodies and add anti-rabbit IgG-HRP conjugate. Wash the plates and add substrate to develop color for 5 min. Read absorbance at 405 nm. The intensity of the colour is directly proportional to the amount of antigen present.
A positive is when the sample signal is double that of the control or pre-immune serum.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Taxol (paclitaxel) is particularly effective as an anticancer agent because tubulin is not its only target. It also binds to a protein called Bcl2, which normally blocks the process of apoptosis, or cell death. Taxol therefore plays a dual role in destroying dividing cells,firstly by stabilising assembled microtubules and thereby halting mitosis, and secondly by inhibiting Bcl2 and allowing apoptosis to proceed.
has not yet been referenced specifically in any publications.
Publishing research using ab26953? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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