Product nameAnti-Taxol antibody
DescriptionRabbit polyclonal to Taxol
Tested applicationsSuitable for: ELISAmore details
Taxol conjugation tg (Thyroglobulin bovine).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab26953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antigen is coated on EIA strips at 0.4 µg per well. Antiserum is diluted in series from 1/10 to 1/10,000 and added in separate wells.
Incubate the antibody for 0.5 hr. Wash off unbound antibodies and add anti-rabbit IgG-HRP conjugate. Wash the plates and add substrate to develop color for 5 min. Read absorbance at 405 nm. The intensity of the colour is directly proportional to the amount of antigen present.
A positive is when the sample signal is double that of the control or pre-immune serum.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTaxol (paclitaxel) is particularly effective as an anticancer agent because tubulin is not its only target. It also binds to a protein called Bcl2, which normally blocks the process of apoptosis, or cell death. Taxol therefore plays a dual role in destroying dividing cells,firstly by stabilising assembled microtubules and thereby halting mitosis, and secondly by inhibiting Bcl2 and allowing apoptosis to proceed.
- Paclitaxel antibody
ab26953 has not yet been referenced specifically in any publications.