Anti-Taxol antibody (ab26953)
Key features and details
- Rabbit polyclonal to Taxol
- Suitable for: ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Taxol antibody -
Description
Rabbit polyclonal to Taxol -
Host species
Rabbit -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Taxol conjugation tg (Thyroglobulin bovine).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | (1) |
Notes |
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Antigen is coated on EIA strips at 0.4 µg per well. Antiserum is diluted in series from 1/10 to 1/10,000 and added in separate wells.
Incubate the antibody for 0.5 hr. Wash off unbound antibodies and add anti-rabbit IgG-HRP conjugate. Wash the plates and add substrate to develop color for 5 min. Read absorbance at 405 nm. The intensity of the colour is directly proportional to the amount of antigen present.
A positive is when the sample signal is double that of the control or pre-immune serum.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Taxol (paclitaxel) is particularly effective as an anticancer agent because tubulin is not its only target. It also binds to a protein called Bcl2, which normally blocks the process of apoptosis, or cell death. Taxol therefore plays a dual role in destroying dividing cells,firstly by stabilising assembled microtubules and thereby halting mitosis, and secondly by inhibiting Bcl2 and allowing apoptosis to proceed. -
Alternative names
- Paclitaxel antibody
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab26953 has been referenced in 2 publications.
- Klein I et al. Impact of drug formulations on kinetics and toxicity in a preclinical model of paclitaxel-induced neuropathy. J Peripher Nerv Syst 26:216-226 (2021). PubMed: 33683765
- Anderson JA et al. In vitro and in vivo evaluation of effect of excipients in local delivery of paclitaxel using microporous infusion balloon catheters. J Biomed Mater Res B Appl Biomater 105:376-390 (2017). PubMed: 26513737