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have you some detail on the sensitivity of Elisa in detecting Paclitaxel
in culture medium by using Anti-Taxol antibody (ab26953)
in alternative have you some references on that
Asked on Oct 10 2012
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The titre of this antibody as observed in indirect ELISA is more than 10,000.
The below table show absorbance at 405nm using 0.4ug of antigen. HRP-conjugated anti rabbit ab was used.
Ab= dilution / Control antiserum / Antiserum ab26953
1:10,000 / 6.769 / 11.442
1:1,000 / 6.402 / 15.622
1:100 /10.650 / 22.400
1:10 /5.216 / 20.468
Antibody is positive at >2 folds of ABS of control/Pre-Immune serum.
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Answered on Oct 10 2012