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  1. Link

    taz-antibody-ab224239.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-TAZ antibody (ab224239)

  • Datasheet
Reviews (1) Submit a question References (13)

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Western blot - Anti-TAZ antibody (ab224239)
  • Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (ab224239)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
  • Western blot - Anti-TAZ antibody (ab224239)

Key features and details

  • Rabbit polyclonal to TAZ
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Protein
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Recombinant Human TAZ protein (ab153252)
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Alexa Fluor® 647 Anti-active YAP1 antibody [EPR19812] (ab225440)

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Overview

  • Product name

    Anti-TAZ antibody
    See all TAZ primary antibodies
  • Description

    Rabbit polyclonal to TAZ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human TAZ aa 1-200.
    Database link: Q9GZV5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: NIH/3T3 and NBT-II cell lysate; human kidney tissue lysate. IHC-P: Human renal cancer, placenta, kidney and colon tissues. ICC/IF: U-251 MG cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Mesenchymal Stem Cells
    • Intracellular

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human TAZ protein (ab153252)
  • Related Products

    • Recombinant Human TAZ protein (ab153252)
    • Recombinant Human TAZ protein (denatured) (ab174421)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab224239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa.
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa.
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
  • Tissue specificity

    Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
  • Sequence similarities

    Contains 1 WW domain.
  • Domain

    The PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
    Binds to transcription factors via its WW domain.
  • Post-translational
    modifications

    Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export.
  • Cellular localization

    Nucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
  • Target information above from: UniProt accession Q9GZV5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 25937 Human
    • Entrez Gene: 97064 Mouse
    • Entrez Gene: 295062 Rat
    • Omim: 607392 Human
    • SwissProt: Q9GZV5 Human
    • SwissProt: Q9EPK5 Mouse
    • Unigene: 535002 Human
    • Unigene: 405029 Mouse
    • Alternative names

      • DKFZP586I1419 antibody
      • FLJ27004 antibody
      • FLJ45718 antibody
      • OTTHUMP00000215994 antibody
      • OTTHUMP00000215995 antibody
      • OTTHUMP00000215996 antibody
      • OTTHUMP00000216001 antibody
      • TAZ antibody
      • Transcriptional co activator with PDZ binding motif antibody
      • Transcriptional coactivator with PDZ binding motif antibody
      • Transcriptional coactivator with PDZ-binding motif antibody
      • WW domain containing transcription regulator 1 antibody
      • WW domain containing transcription regulator protein 1 antibody
      • WW domain-containing transcription regulator protein 1 antibody
      • WWTR 1 antibody
      • WWTR1 antibody
      • WWTR1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TAZ antibody (ab224239)
      Western blot - Anti-TAZ antibody (ab224239)
      Anti-TAZ antibody (ab224239) at 1/250 dilution + Human kidney tissue lysate

      Developed using the ECL technique.

      Predicted band size: 44 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (ab224239)
      Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (ab224239)

      PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for TAZ (green) using ab224239 at 4 µg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)

      Paraffin-embedded human placenta tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)

      Paraffin-embedded human kidney tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)

      Paraffin-embedded human renal cancer tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)

      Paraffin-embedded human colon tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

    • Western blot - Anti-TAZ antibody (ab224239)
      Western blot - Anti-TAZ antibody (ab224239)
      All lanes : Anti-TAZ antibody (ab224239) at 1/250 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
      Lane 2 : NBT-II (rat cell line) cell lysate

      Developed using the ECL technique.

      Predicted band size: 44 kDa

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (13)

    Publishing research using ab224239? Please let us know so that we can cite the reference in this datasheet.

    ab224239 has been referenced in 13 publications.

    • Liu X  et al. Hypoxic bone marrow mesenchymal cell-extracellular vesicles containing miR-328-3p promote lung cancer progression via the NF2-mediated Hippo axis. J Cell Mol Med 25:96-109 (2021). PubMed: 33219752
    • Li W  et al. CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes. Stem Cells Int 2021:6644366 (2021). PubMed: 34394357
    • Liu X  et al. Long Non-Coding RNA MAPK8IP1P2 Inhibits Lymphatic Metastasis of Thyroid Cancer by Activating Hippo Signaling via Sponging miR-146b-3p. Front Oncol 10:600927 (2020). PubMed: 33489905
    • Xie H  et al. miR-1323 suppresses bone mesenchymal stromal cell osteogenesis and fracture healing via inhibiting BMP4/SMAD4 signaling. J Orthop Surg Res 15:237 (2020). PubMed: 32600409
    • Sun GL & Wang D Gallic acid from Terminalia chebula inhibited the growth of esophageal carcinoma cells by suppressing the Hippo signal pathway. Iran J Basic Med Sci 23:1401-1408 (2020). PubMed: 33235697
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TAZ antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Intestinal organoids treated with H202)
    Permeabilization
    Yes - Triton X-100 0.5% in 1X PBS pH 7.4
    Specification
    Intestinal organoids treated with H202
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Mahi Rahman

    Verified customer

    Submitted Jul 31 2021

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