- Datasheet
- Specific References (5)
- Protocols
Overview
-
Product nameAnti-TAZ antibody
See all TAZ primary antibodies -
DescriptionRabbit polyclonal to TAZ
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog
-
Immunogen
Synthetic peptide within Human TAZ aa 125-121 (N terminal). The exact sequence is proprietary.
Sequence:PRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELP
Database link: Q9GZV5 -
Positive control
- Hela cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesPurified by peptide affinity chromatography method.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab84927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
-
FunctionTranscriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
-
Tissue specificityHighly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
-
Sequence similaritiesContains 1 WW domain.
-
DomainThe PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
Binds to transcription factors via its WW domain. -
Post-translational
modificationsPhosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export. -
Cellular localizationNucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
- Information by UniProt
-
Database links
- Entrez Gene: 25937 Human
- Entrez Gene: 97064 Mouse
- Entrez Gene: 295062 Rat
- Omim: 607392 Human
- SwissProt: Q9GZV5 Human
- SwissProt: Q9EPK5 Mouse
- Unigene: 477921 Human
- Unigene: 535002 Human
see all -
Alternative names
- DKFZP586I1419 antibody
- FLJ27004 antibody
- FLJ45718 antibody
see all
Images
-
Western blot - Anti-TAZ antibody (ab84927)All lanes : Anti-TAZ antibody (ab84927) at 1 µg/ml
Lane 1 : HEK293 over-expression
Lane 2 : Empty vector
Lane 3 : Human heart - overexpression
Predicted band size: 44 kDaBlocking buffer used was 3% milk 0.05% TBST
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab84927)IHC image of TAZ staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84927, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (ab84927)This image is courtesy of an anonymous Abreview.Immunocytochemical analysis of N1E-115 murine neuroblastoma cell line, labeling TAZ with ab84927. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X100. Blocking was performed with 4% BSA for 30 minutes at 21°C. Incubation with ab84927 diluted 1/100 in 4% BSA for 45 mintues at 21°C.
-
Western blot - Anti-TAZ antibody (ab84927)This image is courtesy of an anonymous Abreview.Anti-TAZ antibody (ab84927) at 1/1000 dilution + MDA-MB-231 breast cancer cells at 50000 cells with 2% milk + 1% BSA
Performed under reducing conditions.
Predicted band size: 44 kDa
References
This product has been referenced in:
- Abagnale G et al. Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies. Stem Cell Reports 9:654-666 (2017). Read more (PubMed: 28757164) »
- Rice AJ et al. Matrix stiffness induces epithelial-mesenchymal transition and promotes chemoresistance in pancreatic cancer cells. Oncogenesis 6:e352 (2017). ICC/IF . Read more (PubMed: 28671675) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"