Anti-TAZ antibody (ab84927)
- Datasheet
- References (14)
- Protocols
Overview
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Product name
Anti-TAZ antibody
See all TAZ primary antibodies -
Description
Rabbit polyclonal to TAZ -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog
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Immunogen
Synthetic peptide within Human TAZ aa 125-121 (N terminal). The exact sequence is proprietary.
Sequence:PRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELP
Database link: Q9GZV5 -
Positive control
- HeLa whole cell lysate (ab150035) This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by peptide affinity chromatography method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. -
Tissue specificity
Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue. -
Sequence similarities
Contains 1 WW domain. -
Domain
The PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
Binds to transcription factors via its WW domain. -
Post-translational
modificationsPhosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export. -
Cellular localization
Nucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15. - Information by UniProt
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Database links
- Entrez Gene: 25937 Human
- Entrez Gene: 97064 Mouse
- Entrez Gene: 295062 Rat
- Omim: 607392 Human
- SwissProt: Q9GZV5 Human
- SwissProt: Q9EPK5 Mouse
- Unigene: 477921 Human
- Unigene: 535002 Human
see all -
Alternative names
- DKFZP586I1419 antibody
- FLJ27004 antibody
- FLJ45718 antibody
see all
Images
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Western blot - Anti-TAZ antibody (ab84927)All lanes : Anti-TAZ antibody (ab84927) at 1 µg/ml
Lane 1 : HEK293 over-expression
Lane 2 : Empty vector
Lane 3 : Human heart - overexpression
Predicted band size: 44 kDaBlocking buffer used was 3% milk 0.05% TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab84927)IHC image of TAZ staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84927, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (ab84927)This image is courtesy of an anonymous Abreview.Immunocytochemical analysis of N1E-115 murine neuroblastoma cell line, labeling TAZ with ab84927. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X100. Blocking was performed with 4% BSA for 30 minutes at 21°C. Incubation with ab84927 diluted 1/100 in 4% BSA for 45 mintues at 21°C.
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Western blot - Anti-TAZ antibody (ab84927)This image is courtesy of an anonymous Abreview.Anti-TAZ antibody (ab84927) at 1/1000 dilution + MDA-MB-231 breast cancer cells at 50000 cells with 2% milk + 1% BSA
Performed under reducing conditions.
Predicted band size: 44 kDa
Datasheets and documents
References
This product has been referenced in:
- Kingston NM et al. Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells. Methods Mol Biol 1893:107-113 (2019). Read more (PubMed: 30565129) »
- Zhao W et al. Limonin attenuates the stemness of cervical carcinoma cells by promoting YAP nuclear-cytoplasmic translocation. Food Chem Toxicol 125:621-628 (2019). Read more (PubMed: 30738134) »
