Key features and details
- Rabbit Polyclonal to TB Ag85A
- Suitable for: WB, ELISA
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
Product nameAnti-TB Ag85A antibody
See all TB Ag85A primary antibodies
DescriptionRabbit Polyclonal to TB Ag85A
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Recombinant fragment, Other species
Recombinant fragment corresponding to TB Ag85A aa 53-331. (Mycobacterium tuberculosis).
YLQVPSPSMGRDIKVQFQSGGANSPALYLLDGLRAQDDFSGWDINTPAFE WYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKWETFLTSELPGWL QANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAM GPTLIGLAMGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWV YCGNGKPSDLGGNNLPAKFLEGFVRTSNIKFQDAYNAGGGHNGVFDFPDS GTHSWEYWGAQLNAMKPDLQRALGATPNT
Database link: P9WQP2
- Recombinant Mycobacterium tuberculosis TB Ag85A protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notes>95%,Protein G purified
Our Abpromise guarantee covers the use of ab193499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
|ELISA||Use at an assay dependent concentration.|
RelevanceMycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly.
Cellular localizationSecreted; cell wall. Cytoplasm
- 85 A antibody
- 85A antibody
- Ag85A antibody
All lanes : Anti-TB Ag85A antibody (ab193499) at 2 µg/ml
Lane 1 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 1 µg
Lane 2 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 10 µg
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
ab193499 has been referenced in 1 publication.
- Sun L et al. Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice. Biotechnol Lett 39:1159-1166 (2017). WB ; Mouse . PubMed: 28470626