The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Use at an assay dependent concentration.
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly.
Secreted; cell wall. Cytoplasm
85 A antibody
Antigen 85 A antibody
Antigen 85 complex A antibody
Antigen 85A antibody
Fbp A antibody
Fibronectin binding protein A antibody
Mpt 44 antibody
Mycobacterium tuberculosis antigen 85A antibody
Mycolyl transferase 85 A antibody
Mycolyl transferase 85A antibody
Secreted antigen Ag85A antibody
Western blot - Anti-TB Ag85A antibody (ab193499)
All lanes : Anti-TB Ag85A antibody (ab193499) at 2 µg/ml
Lane 1 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 1 µg Lane 2 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 10 µg
Secondary All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa Observed band size: 36 kDa
Sun L et al. Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice. Biotechnol Lett39:1159-1166 (2017).
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