Anti-TB Ag85A antibody (ab193499)
Key features and details
- Rabbit polyclonal to TB Ag85A
- Suitable for: WB, ELISA
- Isotype: IgG
Overview
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Product name
Anti-TB Ag85A antibody
See all TB Ag85A primary antibodies -
Description
Rabbit polyclonal to TB Ag85A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mycobacterium tuberculosis -
Immunogen
Recombinant fragment corresponding to Mycobacterium tuberculosis TB Ag85A aa 50 to the C-terminus.
Database link: P9WQP2 -
Positive control
- Recombinant Mycobacterium tuberculosis TB Ag85A protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
>95%,Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly. -
Cellular localization
Secreted; cell wall. Cytoplasm -
Alternative names
- 85 A antibody
- 85A antibody
- Ag85A antibody
see all
Images
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All lanes : Anti-TB Ag85A antibody (ab193499) at 2 µg/ml
Lane 1 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 1 µg
Lane 2 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 10 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab193499 has been referenced in 1 publication.
- Sun L et al. Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice. Biotechnol Lett 39:1159-1166 (2017). WB ; Mouse . PubMed: 28470626