Key features and details
- Rabbit polyclonal to TBC1D14
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TBC1D14 antibody
DescriptionRabbit polyclonal to TBC1D14
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human TBC1D14 aa 136-435.
DSVKLYSPTSKALTRSDDVSVCSVSSLGTELSTTLSVSNEDILDLVVTSS SSAIVTLENDDDPQFTNVTLSSIKETRGLHQQDCVHEAEEGSKLKILGPF SNFFARNLLARKQSARLDKHNDLGWKLFGKAPLRENAQKDSKRIQKEYED KAGRPSKPPSPKQNVRKNLDFEPLSTTALILEDRPANLPAKPAEEAQKHR QQYEEMVVQAKKRELKEAQRRKKQLEERCRVEESIGNAVLTWNNEILPNW ETMWCSRKVRDLWWQGIPPSVRGKVWSLAIGNELNITHELFDICLARAKE
Database link: Q9P2M4-1
- WB: K562, U-87 MG, A549 and HepG2 whole cell lysate. IHC-P: Human brain and adrenal gland tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IHC-P||1/20 - 1/200.|
FunctionNegative regulator of starvation-induced autophagosome formation.
Sequence similaritiesContains 1 Rab-GAP TBC domain.
Cellular localizationGolgi apparatus, cis-Golgi network. Golgi apparatus, trans-Golgi network. After amino acid starvation, Golgi apparatus-associated protein levels increase compared with fed conditions. May be cycling between the Golgi apparatus and an endosomal pool, redistributing to the Golgi apparatus upon starvation.
- Information by UniProt
- FLJ32400 antibody
- KIAA1322 antibody
- TBC1 domain family member 14 antibody
All lanes : Anti-TBC1D14 antibody (ab235544) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Paraffin-embedded human brain tissue stained for TBC1D14 with ab235544 (1/100 dilution) in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for TBC1D14 with ab235544 (1/100 dilution) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235544 has not yet been referenced specifically in any publications.