Product nameAnti-TBC1D15 antibody
See all TBC1D15 primary antibodies
DescriptionRabbit polyclonal to TBC1D15
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen sequence, corresponding to internal sequence amino acids 169-286 of Human TBC1D15, isoform 2.
- Human testis tissue. RT 4 and U 251 MG cell lysates; Human liver and tonsil tissue lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
FunctionActs as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6.
Sequence similaritiesContains 1 Rab-GAP TBC domain.
- Information by UniProt
- DKFZp686M1379 antibody
- DKFZp761D0223 antibody
- FLJ12085 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121396 at 1/40 dilution staining TBC1D15 in paraffin-embedded Human testis tissue by Immunohistochemistry.
All lanes : Anti-TBC1D15 antibody (ab121396) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
This product has been referenced in:
- Wu J et al. TBC1D15 affects glucose uptake by regulating GLUT4 translocation. Gene 683:210-215 (2019). Read more (PubMed: 30316925) »
- Xian H et al. STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism. Nat Commun 10:2059 (2019). Read more (PubMed: 31053718) »