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    tbc1d15-antibody-ab155035.pdf

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Signal Transduction Second Messenger Nucleotide Messenger GTP
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Anti-TBC1D15 antibody (ab155035)

  • Datasheet
  • SDS
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Western blot - Anti-TBC1D15 antibody (ab155035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab155035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab155035)

Key features and details

  • Rabbit polyclonal to TBC1D15
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-TBC1D15 antibody
    See all TBC1D15 primary antibodies
  • Description

    Rabbit polyclonal to TBC1D15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 110-348 of Human TBC1D15 (Uniprot ID: Q8TC07).

  • Positive control

    • 293T, A431, HeLa and HepG2 whole cell lysates, Mouse gastric carcinoma and Human muscle tissues
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 79 kDa.
IHC-P
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 79 kDa.
IHC-P
1/100 - 1/1000.

Target

  • Function

    Acts as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6.
  • Sequence similarities

    Contains 1 Rab-GAP TBC domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q8TC07 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64786 Human
    • Entrez Gene: 66687 Mouse
    • Omim: 612662 Human
    • SwissProt: Q8TC07 Human
    • SwissProt: Q9CXF4 Mouse
    • Unigene: 284630 Human
    • Unigene: 22252 Mouse
    • Alternative names

      • DKFZp686M1379 antibody
      • DKFZp761D0223 antibody
      • FLJ12085 antibody
      • FLJ33191 antibody
      • GAP for RAB7 antibody
      • GTPase-activating protein RAB7 antibody
      • RAB7 GAP antibody
      • Rab7-GAP antibody
      • TBC1 domain family member 15 antibody
      • TBC15_HUMAN antibody
      • Tbc1d15 antibody
      • Tre 2 budding uninhibited by benzimidazole cell division cycle 16 domain, domain family member 15 antibody
      • Tre2/Bub2/Cdc16 domain family member 15 antibody
      see all

    Images

    • Western blot - Anti-TBC1D15 antibody (ab155035)
      Western blot - Anti-TBC1D15 antibody (ab155035)
      All lanes : Anti-TBC1D15 antibody (ab155035) at 1/3000 dilution

      Lane 1 : 293T whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 79 kDa



      7.5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab155035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab155035)
      Immunohistochemical analysis of paraffin-embedded Mouse gastric carcinoma, labeling TBC1D15 using ab155035 at a 1/500 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab155035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab155035)
      Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling TBC1D15 using ab155035 at a 1/500 dilution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab155035? Please let us know so that we can cite the reference in this datasheet.

    ab155035 has not yet been referenced specifically in any publications.

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