Product nameAnti-TBC1D15 antibody - N-terminal
See all TBC1D15 primary antibodies
DescriptionRabbit polyclonal to TBC1D15 - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Cat, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab194953 was affinity purified using an epitope specific to TBC1D15 immobilized on solid support.
Our Abpromise guarantee covers the use of ab194953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 79 kDa.|
FunctionActs as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6.
Sequence similaritiesContains 1 Rab-GAP TBC domain.
- Information by UniProt
- DKFZp686M1379 antibody
- DKFZp761D0223 antibody
- FLJ12085 antibody
All lanes : Anti-TBC1D15 antibody - N-terminal (ab194953) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 30 seconds
Whole cell lysates prepared using NETN lysis buffer.
Detection of TBC1D15 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194953 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab194953 has not yet been referenced specifically in any publications.