Anti-TBC1D15 antibody - N-terminal (ab194953)

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Overview

  • Product name

    Anti-TBC1D15 antibody - N-terminal
    See all TBC1D15 primary antibodies

  • Description

    Rabbit polyclonal to TBC1D15 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Cat, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan

  • Immunogen

    Synthetic peptide within Human TBC1D15 aa 1-50 (N terminal). The exact sequence is proprietary. (NP_073608.4).
    Sequence:

    MAAAGVVSGKIIYEQEGVYIHSSCGKTNDQDGLISGILRVLEKDAEVIVD


    Database link: Q8TC07

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab194953 was affinity purified using an epitope specific to TBC1D15 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 79 kDa.

Target

Images

  • Western blot - Anti-TBC1D15 antibody - N-terminal (ab194953)
    Western blot - Anti-TBC1D15 antibody - N-terminal (ab194953)
    All lanes : Anti-TBC1D15 antibody - N-terminal (ab194953) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH/3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 79 kDa


    Exposure time: 30 seconds


    Whole cell lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-TBC1D15 antibody - N-terminal (ab194953)
    Immunoprecipitation - Anti-TBC1D15 antibody - N-terminal (ab194953)

    Detection of TBC1D15 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194953 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab194953 has not yet been referenced specifically in any publications.

Publishing research using ab194953? Please let us know so that we can cite the reference in this datasheet.

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