Product nameAnti-TBC1D2 antibody - N-terminal
See all TBC1D2 primary antibodies
DescriptionRabbit polyclonal to TBC1D2 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
- Recombinant Human TBC1D2 protein (ab163081) can be used as a positive control in WB. HeLa, 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 105 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceActs as GTPase-activating protein for RAB7A. Signal effector acting as a linker between RAC1 and RAB7A, leading to RAB7A inactivation and subsequent inhibition of cadherin degradation and reduced cell-cell adhesion. Interacts with activated RAC1 and CDH1.
Cellular localizationCytoplasm. Cytoplasmic vesicle. Cell junction
- PARIS 1 antibody
- PARIS1 antibody
- PP8997 antibody
All lanes : Anti-TBC1D2 antibody - N-terminal (ab176701) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds
Detection of TBC1D2 by Western Blot of Immunprecipitate.
Western blot using ab176701 at 0.4 µg/ml staining TBC1D2 in HeLa whole cell lysate immunoprecipitated using ab176701 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure times of 3 seconds
ab176701 has not yet been referenced specifically in any publications.