Anti-TBC1D20 antibody (ab237685)
Key features and details
- Rabbit polyclonal to TBC1D20
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TBC1D20 antibody -
Description
Rabbit polyclonal to TBC1D20 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human TBC1D20 aa 1-237.
Database link: Q96BZ9 -
Positive control
- WB: Mouse brain and stomach lysates. IHC-P: Human testis and adrenal gland tissue. ICC/IF: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab237685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. | |
| WB | 1/500 - 1/5000. Predicted molecular weight: 46 kDa. |
Target
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Function
May act as a GTPase-activating protein for Rab family protein(s). -
Sequence similarities
Contains 1 Rab-GAP TBC domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 128637 Human
- Entrez Gene: 67231 Mouse
- Omim: 611663 Human
- SwissProt: Q96BZ9 Human
- SwissProt: Q9D9I4 Mouse
- Unigene: 590876 Human
- Unigene: 288733 Mouse
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Alternative names
- 1110028I04Rik antibody
- 2810442O16Rik antibody
- AI414693 antibody
see all
Images
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Western blot - Anti-TBC1D20 antibody (ab237685)All lanes : Anti-TBC1D20 antibody (ab237685) at 1/500 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse stomach lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D20 antibody (ab237685)Paraffin-embedded human testis tissue stained for TBC1D20 using ab237685 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D20 antibody (ab237685)Paraffin-embedded human adrenal gland tissue stained for TBC1D20 using ab237685 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TBC1D20 antibody (ab237685)MCF7 (human breast adenocarcinoma cell line) cells stained for TBC1D20 (green) using ab237685 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab237685 has not yet been referenced specifically in any publications.
