Key features and details
- Rabbit polyclonal to TBC1D9B - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TBC1D9B antibody - C-terminal
See all TBC1D9B primary antibodies
DescriptionRabbit polyclonal to TBC1D9B - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human TBC1D9B aa 1200-1250 (C terminal). The exact sequence is proprietary.
Database link: Q66K14
- HeLa, 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab200578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 141 kDa.|
FunctionMay act as a GTPase-activating protein for Rab family protein(s).
Sequence similaritiesContains 1 EF-hand domain.
Contains 2 GRAM domains.
Contains 1 Rab-GAP TBC domain.
- Information by UniProt
- KIAA0676 antibody
- TBC1 domain family member 9B antibody
- TBC1 domain family, member 9B (with GRAM domain) antibody
All lanes : Anti-TBC1D9B antibody - C-terminal (ab200578) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 141 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling TBC1D9B using ab200578 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated TBC1D9B, ab200578 was used at 1 μg/ml.
ab200578 has not yet been referenced specifically in any publications.