Overview

  • Product name

  • Description

    Rabbit polyclonal to TBCD/SSD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TBCD/SSD1 aa 2-96.
    Database link: Q9BTW9

  • Positive control

    • IHC-P: Human cervical cancer tissue. ICC/IF: A549 cells.
  • General notes

     This product was previously labelled as TBCD

     

Properties

Applications

Our Abpromise guarantee covers the use of ab238761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Tubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Acts as a GTPase-activating protein (GAP) for ARL2. Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the TBCD family.
    Contains 3 HEAT repeats.
  • Cellular localization

    Cell junction > tight junction. Lateral cell membrane. Cytoplasm. Cell junction > adherens junction. Localized in cell-cell contacts.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta tubulin cofactor D antibody
    • Beta-tubulin cofactor D antibody
    • KIAA0988 antibody
    • SSD 1 antibody
    • SSD-1 antibody
    • SSD1 antibody
    • tbcd antibody
    • TBCD_HUMAN antibody
    • tfcD antibody
    • Tubulin folding cofactor D antibody
    • Tubulin specific chaperone D antibody
    • Tubulin-folding cofactor D antibody
    • Tubulin-specific chaperone D antibody
    see all

Images

  • Paraffin-embedded human cervical cancer tissue stained for TBCD/SSD1 using ab238761 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells stained for TBCD/SSD1 (green) using ab238761 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

References

ab238761 has not yet been referenced specifically in any publications.

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