Product nameAnti-TBL1 antibody
See all TBL1 primary antibodies
DescriptionRabbit polyclonal to TBL1
SpecificityDoes not cross-react with TBLR1.
Tested applicationsSuitable for: ChIP, WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein (Human)
- HeLa nuclear extract
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
ChIP Related Products
Our Abpromise guarantee covers the use of ab24548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent dilution.|
|WB||1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).|
|IP||Use at an assay dependent dilution.|
FunctionF-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
Sequence similaritiesBelongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats.
DomainThe F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
- Information by UniProt
- EBI antibody
- F box like/WD repeat protein TBL1X antibody
- F-box-like/WD repeat-containing protein TBL1X antibody
Anti-TBL1 antibody (ab24548) at 1/1000 dilution + HeLa nuclear extract.
Predicted band size: 63 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
TBL1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TBL1 - ChIP Grade (ab24548) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab24548.
Secondary: Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 64kDa: TBL1.
ab24548 staining TBL1 (green) in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
This product has been referenced in:
- Guy J et al. A mutation-led search for novel functional domains in MeCP2. Hum Mol Genet 27:2531-2545 (2018). Read more (PubMed: 29718204) »
- Stoy C et al. Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy. EMBO Mol Med 7:1048-62 (2015). ChIP . Read more (PubMed: 26070712) »