Product nameAnti-TBP-1 antibody
See all TBP-1 primary antibodies
DescriptionRabbit polyclonal to TBP-1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human TBP-1 aa 389-439.
- 293T, NIH 3T3, Jurkat and HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
- HeLa nuclear extract lysate (ab14655)
- Jurkat cytoplasmic extract lysate (ab14842)
- Jurkat nuclear extract lysate (ab14844)
- NIH 3T3 cytoplasmic extract lysate (ab14873)
- NIH 3T3 nuclear extract lysate (ab14874)
- HeLa whole cell lysate (ab29545)
- NIH 3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
Our Abpromise guarantee covers the use of ab125164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
Sequence similaritiesBelongs to the AAA ATPase family.
modificationsSumoylated by UBE2I in response to MEKK1-mediated stimuli.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 26S protease regulatory subunit 6A antibody
- 26S proteasome AAA-ATPase subunit RPT5 antibody
- Human immunodeficiency virus tat transactivator binding protein 1 antibody
All lanes : Anti-TBP-1 antibody (ab125164) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds
ab125164, at 6 µg/mg lysate, staining TBP-1 in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane).
For the subsequent Western blot, ab125164 was used at 1 µg/ml, and the blot developed using the ECL technique, with an exposure time of 10 seconds.
ab125164 has not yet been referenced specifically in any publications.