Key features and details
- Rabbit polyclonal to TBP-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TBP-1 antibody
See all TBP-1 primary antibodies
DescriptionRabbit polyclonal to TBP-1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human TBP-1 aa 389-439.
- 293T, NIH 3T3, Jurkat and HeLa whole cell lysate (ab150035)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
Sequence similaritiesBelongs to the AAA ATPase family.
modificationsSumoylated by UBE2I in response to MEKK1-mediated stimuli.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 26S protease regulatory subunit 6A antibody
- 26S proteasome AAA-ATPase subunit RPT5 antibody
- Human immunodeficiency virus tat transactivator binding protein 1 antibody
All lanes : Anti-TBP-1 antibody (ab125164) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds
ab125164, at 6 µg/mg lysate, staining TBP-1 in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane).
For the subsequent Western blot, ab125164 was used at 1 µg/ml, and the blot developed using the ECL technique, with an exposure time of 10 seconds.
ab125164 has not yet been referenced specifically in any publications.