Key features and details
- Rabbit polyclonal to TBP-1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TBP-1 antibody
See all TBP-1 primary antibodies
DescriptionRabbit polyclonal to TBP-1
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human TBP-1 aa 1-300.
MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDS EIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVD PNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDL VGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVE AIVLPMNHKEKFENLGIQPPKGVLMYGPPGTGKTLLARACAAQTKATFLK LAGPQLVQMFIGDGAKLVRDAFALAKEKAPSIIFIDELDAIGTKRFDSEK
Database link: P17980
- IHC-P: Human lung cancer and testis tissues. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab239029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|IHC-P||1/20 - 1/200.|
FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
Sequence similaritiesBelongs to the AAA ATPase family.
modificationsSumoylated by UBE2I in response to MEKK1-mediated stimuli.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 26S protease regulatory subunit 6A antibody
- 26S proteasome AAA-ATPase subunit RPT5 antibody
- Human immunodeficiency virus tat transactivator binding protein 1 antibody
A549 (human lung carcinoma cell line) cells stained for TBP-1 (green) using ab239029 at 1/530 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain was DAPI (blue).
Paraffin-embedded human testis tissue stained for TBP-1 using ab239029 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human lung cancer tissue stained for TBP-1 using ab239029 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239029 has not yet been referenced specifically in any publications.