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Synthetic peptide within Human TBP-1 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P17980
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab171969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
Western blot analysis on immunoprecipitation pellet from (Lane 1) HeLa cell lysate or (Lane 2) 1X PBS (negative control) using ab171969 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling TBP-1 using ab171969 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling TBP-1 using ab171969 at a 1/50 dilution.
Immunofluorescence analysis of 293 cells labeling TBP-1 using ab171969 at a 1/250 dilution.
Flow cytometric analysis of permeabilized 293 cells, labeling TBP-1 using ab171969 at a 1/10 dilution (red) or a rabbit IgG negative control (green).
ab171969 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"