Overview

  • Product name
    Anti-TBP-1 antibody [EPR12153]
    See all TBP-1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12153] to TBP-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TBP-1 aa 400 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P17980

  • Positive control
    • 293T, HeLa and HepG2 cell lysates; Human pancreas tissue; Human Tonsil tissue; Permeabilized HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12153
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
    • Sequence similarities
      Belongs to the AAA ATPase family.
    • Post-translational
      modifications
      Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26S protease regulatory subunit 6A antibody
      • 26S proteasome AAA-ATPase subunit RPT5 antibody
      • Human immunodeficiency virus tat transactivator binding protein 1 antibody
      • MGC8487 antibody
      • Proteasome (prosome macropain) 26S subunit ATPase 3 antibody
      • Proteasome 26S ATPase subunit 3 antibody
      • Proteasome 26S subunit ATPase 3 antibody
      • Proteasome subunit P50 antibody
      • PRS6A_HUMAN antibody
      • PSMC 3 antibody
      • PSMC3 antibody
      • Tat binding protein 1 antibody
      • TAT-binding protein 1 antibody
      • TBP-1 antibody
      • TBP1 antibody
      see all

    Images

    • All lanes :

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TBP-1 with ab171974 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TBP-1 with ab171974 at 1/100 dilution

    • Flow cytometric analysis of permeabilized HeLa cells labeling TBP-1 with ab171974 at 1/100 dilution (red) or a Rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Luan Y  et al. Chronic Caffeine Treatment Protects Against a-Synucleinopathy by Reestablishing Autophagy Activity in the Mouse Striatum. Front Neurosci 12:301 (2018). Read more (PubMed: 29770111) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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