Key features and details
- Rabbit Polyclonal to Tbp7
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Tbp7 antibody
See all Tbp7 primary antibodies
DescriptionRabbit Polyclonal to Tbp7
SpecificityThis antibody detects endogenous level of total Tbp7 protein.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human Tbp7 (N terminal).
Database link: P43686
- U2OS cells. Rat testis, human kidney and human breast cancer tissue. Cell lysates HepG2, U251, 293T, BT474, A549, HeLa, Jurkat and mouse liver.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 47 kDa.|
FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Sequence similaritiesBelongs to the AAA ATPase family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 26S protease regulatory subunit 6B antibody
- 26S proteasome AAA ATPase subunit RPT3 antibody
- 26S proteasome AAA-ATPase subunit RPT3 antibody
All lanes : Anti-Tbp7 antibody (ab196589) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : U251 cell extract
Lane 3 : 293T cell extract
Lane 4 : BT474 cell extract
Lane 5 : A549 cell extract
Lane 6 : HeLa cell extract
Lane 7 : Jurkat cell extract
Lanes 8-9 : Mouse liver extract
Predicted band size: 47 kDa
Immunohistochemical analysis of paraffin-embedded rat testis tissue labelling Tbp7 using ab196589 at a 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Tbp7 using ab196589 at a 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labelling Tbp7 using ab196589 at a 1/100 dilution.
Immunofluorescence analysis of U2OS cell labelling Tbp7 using ab196589 at a 1/50 dilution (top) with DAPI blue for nuclear staining (bottom)
ab196589 has not yet been referenced specifically in any publications.