Overview

  • Product name

  • Description

    Rabbit Polyclonal to Tbp7
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous level of total Tbp7 protein.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Tbp7 (N terminal).
    Database link: P43686

  • Positive control

    • U2OS cells. Rat testis, human kidney and human breast cancer tissue. Cell lysates HepG2, U251, 293T, BT474, A549, HeLa, Jurkat and mouse liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab196589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 47 kDa.

Target

  • Function

    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S protease regulatory subunit 6B antibody
    • 26S proteasome AAA ATPase subunit RPT3 antibody
    • 26S proteasome AAA-ATPase subunit RPT3 antibody
    • MB67 interacting protein antibody
    • MB67-interacting protein antibody
    • MIP224 antibody
    • Protease 26S subunit 6 antibody
    • Proteasome (prosome macropain) 26S subunit ATPase 4 antibody
    • Proteasome 19S S6 antibody
    • Proteasome 26S subunit ATPase 4 antibody
    • Proteasome 26S subunit, ATPase, 4 antibody
    • PRS6B_HUMAN antibody
    • PSMC4 antibody
    • RPT3 antibody
    • S6 antibody
    • Tat binding protein 7 antibody
    • TAT-binding protein 7 antibody
    • TBP 7 antibody
    • TBP-7 antibody
    see all

Images

  • All lanes : Anti-Tbp7 antibody (ab196589) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : U251 cell extract
    Lane 3 : 293T cell extract
    Lane 4 : BT474 cell extract
    Lane 5 : A549 cell extract
    Lane 6 : HeLa cell extract
    Lane 7 : Jurkat cell extract
    Lanes 8-9 : Mouse liver extract

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue labelling Tbp7 using ab196589 at a 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Tbp7 using ab196589 at a 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labelling Tbp7 using ab196589 at a 1/100 dilution.

  • Immunofluorescence analysis of U2OS cell labelling Tbp7 using ab196589 at a 1/50 dilution (top) with DAPI blue for nuclear staining (bottom)

References

ab196589 has not yet been referenced specifically in any publications.

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