Key features and details
- Rabbit polyclonal to Tbp7
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-Tbp7 antibody
See all Tbp7 primary antibodies
DescriptionRabbit polyclonal to Tbp7
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Mouse, Cow, Cynomolgus monkey
Recombinant full length protein corresponding to Human Tbp7 aa 1-418. (Expressed in E.coli).
MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQE LEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNT AIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSS IMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPR GVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDV FRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFD QNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITS KMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFE KAYKTVIKKDEQEHEFYK
Database link: P43686
- IHC-P: Human prostate and kidney tissues. WB: Recombinant human Tbp7 protein; Human kidney lysate; Jurkat cell lysate; Pig skeletal muscle and cerebrum lysate; Rat testis lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 47 kDa.|
FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Sequence similaritiesBelongs to the AAA ATPase family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 26S protease regulatory subunit 6B antibody
- 26S proteasome AAA ATPase subunit RPT3 antibody
- 26S proteasome AAA-ATPase subunit RPT3 antibody
All lanes : Anti-Tbp7 antibody (ab233194) at 1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Pig muscle tissue lysate
Lane 3 : Pig brain tissue lysate
Lane 4 : Rat testis tissue lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Anti-Tbp7 antibody (ab233194) at 2 µg/ml + Recombinant human Tbp7 protein.
Predicted band size: 47 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for Tbp7 using ab233194 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate gland tissue stained for Tbp7 using ab233194 at 20 µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233194 has not yet been referenced specifically in any publications.