Recombinant Anti-Tbp7 antibody [EPR9911(B)] (ab139184)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9911(B)] to Tbp7
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Tbp7 antibody [EPR9911(B)]
See all Tbp7 primary antibodies -
Description
Rabbit monoclonal [EPR9911(B)] to Tbp7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Tbp7 aa 400-500 (C terminal). The exact sequence is proprietary.
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Positive control
- HepG2, HeLa, 293T and U87-MG cell lysates; U87-MG cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9911(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. -
Sequence similarities
Belongs to the AAA ATPase family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5704 Human
- Entrez Gene: 23996 Mouse
- Entrez Gene: 117262 Rat
- Omim: 602707 Human
- SwissProt: P43686 Human
- SwissProt: P54775 Mouse
- SwissProt: Q63570 Rat
- Unigene: 211594 Human
see all -
Alternative names
- 26S protease regulatory subunit 6B antibody
- 26S proteasome AAA ATPase subunit RPT3 antibody
- 26S proteasome AAA-ATPase subunit RPT3 antibody
see all
Images
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All lanes : Anti-Tbp7 antibody [EPR9911(B)] (ab139184) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa -
Immunofluorescent analysis of U87-MG cells labelling Tpb7 with ab139184 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab139184 has been referenced in 1 publication.
- Alber AB et al. Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates. Mol Cell 71:1079-1091.e9 (2018). PubMed: 30146318