Anti-TBR1 antibody (ab31940)
Key features and details
- Rabbit polyclonal to TBR1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TBR1 antibody
See all TBR1 primary antibodies -
Description
Rabbit polyclonal to TBR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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IHC-P | (6) |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 10716 Human
- Entrez Gene: 21375 Mouse
- Entrez Gene: 680427 Rat
- Omim: 604616 Human
- SwissProt: Q16650 Human
- SwissProt: Q64336 Mouse
- Unigene: 210862 Human
- Unigene: 308525 Mouse
see all -
Alternative names
- T box brain 1 antibody
- T box brain protein 1 antibody
- T brain 1 protein antibody
see all
Images
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All lanes : Anti-TBR1 antibody (ab31940) at 1 µg/ml
Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Rat hippocampus tissue lysate
Lane 3 : Human hippocampus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 4 minutesGel type: MOPS
Blocking buffer: 3% milk block
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IHC image of TBR1 staining in a section of formalin-fixed paraffin-embedded normal E17 mouse brain performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab31940, 1/2000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
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All lanes : Anti-TBR1 antibody (ab31940) at 1 µg/ml
Lane 1 : Mouse hippocampus whole cell lysate
Lane 2 : Rat hippocampus whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab31940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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IHC-P image of TBR1 staining on mouse brain sections using ab31940 at a 1:200 dilution.
The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were blocked using 7.5% goat serum for 2 hours at room temperature. ab31940 was diluted 1:200 using blocking buffer and incubated with the sections for 16 hours at 4°C. The secondary antibody used was Goat polyclonal to anti-rabbit conjugated to Alexa Fluor® 594 (1:400).
DAPI was used to counterstain nuclei.
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(Image courtesy of Human Protein Atlas)
ab31940 staining TBR1 protein in normal human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab31940 at a 1/25 dilution for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (425)
ab31940 has been referenced in 425 publications.
- Junaković A et al. Laminar dynamics of deep projection neurons and mode of subplate formation are hallmarks of histogenetic subdivisions of the human cingulate cortex before onset of arealization. Brain Struct Funct 228:613-633 (2023). PubMed: 36592215
- Han X et al. Loss of RNA-Binding Protein HuR Leads to Defective Ependymal Cells and Hydrocephalus. J Neurosci 42:202-219 (2022). PubMed: 34815315
- Wei C et al. RhoGEF Trio Regulates Radial Migration of Projection Neurons via Its Distinct Domains. Neurosci Bull 38:249-262 (2022). PubMed: 34914033
- Schembs L et al. The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling. Cell Rep 39:110811 (2022). PubMed: 35584663
- Rosebrock D et al. Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol 24:981-995 (2022). PubMed: 35697781