Key features and details
- Rabbit polyclonal to TBR1
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-FoFr, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TBR1 antibody
See all TBR1 primary antibodies
DescriptionRabbit polyclonal to TBR1
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-FoFr, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
- IHC-P: Mouse E17 brain tissue. Human cerebral cortex tissue. IHC-Fr: Mouse and rat brain tissue. ICC/IF: Rat cortical neurons. WB: Mouse and rat hippocampus whole cell lysate. Flow Cyt: Human ES cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab31940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Mouse TBR1 peptide (ab25853).|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19709629|
|IHC-P||1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- Information by UniProt
- T box brain 1 antibody
- T box brain protein 1 antibody
- T brain 1 protein antibody
IHC image of TBR1 staining in a section of formalin-fixed paraffin-embedded normal E17 mouse brain performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab31940, 1/2000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
ab31940 staining mouse brain tissue by IHC-Fr.
The sections were fixed with PFA and permeabilized in PBST. The primary antibody was diluted 1/500 and incubated with the sample for 14 hours. An Alexa Fluor® 594 goat anti-rabbit antibody was used as the secondary.
Human iPSCs (induced pluripotent stem cell) were fixed with 4% paraformaldehyde, followed by membrane permeabilization and blocking with 0.1% Triton X-100 in donkey serum. Samples were incubated with ab31940 at a 1:200 dilution overnight then with the secondary antibody for 1 hour. Imaging was performed using a Zeiss LSM710 confocal microscope and images were acquired using ZEN black software. Software was used to pseudo-color images and add scale bars.
Quantified MAP2 immunostaining was performed blind on at least 3 images per condition, with at least 200 cells counted per image, using ImageJ software (NIH).
IHC-P image of TBR1 staining on mouse brain sections using ab31940 at a 1:200 dilution.
The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were blocked using 7.5% goat serum for 2 hours at room temperature. ab31940 was diluted 1:200 using blocking buffer and incubated with the sections for 16 hours at 4°C. The secondary antibody used was Goat polyclonal to anti-rabbit conjugated to Alexa Fluor® 594 (1:400).
DAPI was used to counterstain nuclei.
ab31940 staining rat brain sections by IHC-Fr.
The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. The tissue was blocked with 10% donkey serum in 0.3% TritonX in 0.1% PBS for 30 minutes at 24°C. Staining with ab31940 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS- 10% donkey serum was performed for 4 hours at 24°C. A donkey anti-rabbit Alexa-Fluor® 488 polyclonal antibody at 1/1000 was used as the secondary antibody.
TBR1 expressed in the several cortical layers distinctly in pyramidal neurons.
(Image courtesy of Human Protein Atlas)
ab31940 staining TBR1 protein in normal human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab31940 at a 1/25 dilution for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31940 staining rat cortical neurons by ICC.
The cultured cells were fixed with PFA and permeabilized in 0.1% Triton X prior to blocking in 10% BSA for 1 hour at 22°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 22°C. A Cy2® donkey anti-rabbit antibody was used as the secondary
All lanes : Anti-TBR1 antibody (ab31940) at 1 µg/ml
Lane 1 : Mouse hippocampus whole cell lysate
Lane 2 : Rat hippocampus whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab31940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab31940 staining TBR1 in human ES cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with Triton X-100. The sample was incubated with the primary antibody (1/200) for 30 minutes. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/20000) was used as the secondary antibody. Gating Strategy: All cells.
ab31940 has been referenced in 250 publications.
- Frew J et al. Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency. Mol Neurodegener 15:21 (2020). PubMed: 32178712
- Güven A et al. Extracellular matrix-inducing Sox9 promotes both basal progenitor proliferation and gliogenesis in developing neocortex. Elife 9:N/A (2020). PubMed: 32191207
- Hattori Y et al. Transient microglial absence assists postmigratory cortical neurons in proper differentiation. Nat Commun 11:1631 (2020). PubMed: 32242005
- Stathakos P et al. Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research. Methods Mol Biol 1880:257-280 (2019). PubMed: 30610703
- Forbes LH & Andrews MR Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract. Front Cell Neurosci 13:26 (2019). PubMed: 30809126