Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
- Datasheet
- Specific References (3)
- Protocols
Overview
-
Product nameAnti-TBR2 / Eomes antibody [EPR19012]
See all TBR2 / Eomes primary antibodies -
DescriptionRabbit monoclonal [EPR19012] to TBR2 / Eomes
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
-
Species reactivityReacts with: Mouse
Does not react with: Human -
Immunogen
Recombinant fragment within Mouse TBR2/ Eomes aa 250-500. The exact sequence is proprietary.
Database link: O54839 -
Positive control
- IHC-P: Mouse hippocampus and E14 cortex tissues. IHC-Fr: Mouse E14.5 cortex and hippocampus tissues.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR19012
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab183991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/1000. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
|
| IHC-P | 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
FunctionFunctions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
-
Tissue specificityExpressed in CD8+ T-cells.
-
Involvement in diseaseNote=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.
-
Sequence similaritiesContains 1 T-box DNA-binding domain.
-
Developmental stageDetected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.
-
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 13813 Mouse
- SwissProt: O54839 Mouse
- Unigene: 200692 Mouse
-
Alternative names
- eomes antibody
- EOMES_HUMAN antibody
- Eomesodermin antibody
see all
Images
-
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266944-183991IHCFra.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on E14.5 mouse cortex is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266946-183991IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on intermediate neuronal progenitors of mouse hippocampus is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266945-183991IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of paraffin-embedded mouse E14 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on the cortex of mouse E14 is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266943-183991IHCFrb.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on adult mouse hippocampus is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266942-183991IHCFrc.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen adult mouse kidney tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Negative control tissue; very weak staining is due to autoflorescence of the kidney section.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Protocols
References
This product has been referenced in:
- Stefani J et al. Disruption of the Microglial ADP Receptor P2Y13 Enhances Adult Hippocampal Neurogenesis. Front Cell Neurosci 12:134 (2018). Read more (PubMed: 29867367) »
- Huang X et al. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling. Elife 6:N/A (2017). Read more (PubMed: 29168693) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"