Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Adult dentate gyrus)
Specification
Adult dentate gyrus
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS +TritonX-100(0.05%) Donkey serum(3%)
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit Thermo fisher
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Animals were perfused with 4% PFA. Fixed over night in 4% PFA. Placed in 30% sucrose for 48 hours. 45micron thick sections were done on microtome.
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Submitted Dec 09 2016