Immunohistochemistry (Frozen sections) abreview for Anti-TBR2 / Eomes antibody [EPR19012]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain / DG)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Brain / DG
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 0.2% Triton X-100
Dilution
1/250

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG (H+L) ML (Jackson)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

Additional data

Additional Notes
Frozen brain sections from p21 mouse. Tbr2+ neural progenitor cells (red) in SGZ.

Wei Mo

Verified customer

Submitted Dec 22 2016

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