Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TBR2 / Eomes antibody [EPR19012]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryonic mouse brain coronal tissue section E.17)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer
Permeabilization
Yes - Triton-X-100
Specification
Embryonic mouse brain coronal tissue section E.17
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Carnoy's solution

Other product details

Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/800

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Molecular Probes (A11012)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

Additional data

Additional Notes
This image shows E.17 coronal brain section which shows Tbr2 positive cells (red) in SVZ and VZ. The sections were antigen retrieved using 10mM Sodium Citrate buffer (pH 6) for 10 mins at 650W in a microwave.

Abcam user community

Verified customer

Submitted Apr 18 2017

Sign up