Recombinant
RabMAb

Recombinant Anti-TBR2 / Eomes antibody [EPR19012] (Alexa Fluor® 568) (ab222324)

Overview

  • Product name

    Anti-TBR2 / Eomes antibody [EPR19012] (Alexa Fluor® 568)
    See all TBR2 / Eomes primary antibodies
  • Description

    Rabbit monoclonal [EPR19012] to TBR2 / Eomes (Alexa Fluor® 568)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Tested applications

    Suitable for: IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse TBR2/ Eomes aa 250-500. The exact sequence is proprietary.
    Database link: O54839

  • Positive control

    • IHC-Fr: fetal mouse E14.5 brain tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.

Antigen Retrieval: heat mediated by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Target

  • Function

    Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
  • Tissue specificity

    Expressed in CD8+ T-cells.
  • Involvement in disease

    Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Developmental stage

    Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • eomes antibody
    • EOMES_HUMAN antibody
    • Eomesodermin antibody
    • Eomesodermin homolog antibody
    • t box brain 2 antibody
    • T-box brain protein 2 antibody
    • T-brain-2 antibody
    • Tbr 2 antibody
    • TBR-2 antibody
    see all

Images

  • IHC image of TBR2 / Eomes staining in a section of frozen fetal mouse E14.5 brain.

    The section was fixed using 4% paraformaldehyde and permeabilized using 0.2% Triton X-100. The section was pre-treated using heat mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab222324 at 1/100 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

References

This product has been referenced in:

  • Wezel A  et al. Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response. Sci Rep 6:24248 (2016). Read more (PubMed: 27053419) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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