RecombinantRabMAb

Recombinant Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunocytochemistry - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19012] to TBR2 / Eomes - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P, ICC
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free
    See all TBR2 / Eomes primary antibodies

  • Description

    Rabbit monoclonal [EPR19012] to TBR2 / Eomes - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human

  • Immunogen

    Recombinant fragment aa 250-500. The exact sequence is proprietary.
    Database link: O54839

  • Positive control

    • IHC-P: Mouse hippocampus and E14 cortex tissues; IHC-Fr: Mouse E14.5 cortex and hippocampus tissues; ICC: Mouse primary neuron cells.

  • General notes

    Ab222226 is the carrier-free version of ab183991. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab222226 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab222226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • Function

    Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.

  • Tissue specificity

    Expressed in CD8+ T-cells.

  • Involvement in disease

    Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.

  • Sequence similarities

    Contains 1 T-box DNA-binding domain.

  • Developmental stage

    Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • eomes antibody
      • EOMES_HUMAN antibody
      • Eomesodermin antibody
      • Eomesodermin homolog antibody
      • t box brain 2 antibody
      • T-box brain protein 2 antibody
      • T-brain-2 antibody
      • Tbr 2 antibody
      • TBR-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on intermediate neuronal progenitors of mouse hippocampus is observed [PMID: 22553033]. 

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunocytochemistry - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with ab183991 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Specific staining on E14.5 mouse cortex is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of paraffin-embedded mouse E14 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on the cortex of mouse E14 is observed [PMID: 22553033]. 

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Specific staining on adult mouse hippocampus is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen adult mouse kidney tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Negative control tissue; very weak staining is due to autoflorescence of the kidney section.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

    References (0)

    Publishing research using ab222226? Please let us know so that we can cite the reference in this datasheet.

    ab222226 has not yet been referenced specifically in any publications.

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