Recombinant
RabMAb

Recombinant Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Overview

  • Product name

    Anti-TBR2 / Eomes antibody [EPR21950-241]
    See all TBR2 / Eomes primary antibodies
  • Description

    Rabbit monoclonal [EPR21950-241] to TBR2 / Eomes
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TBR2/ Eomes aa 550-650. The exact sequence is proprietary.
    Database link: O95936

  • Positive control

    • IHC-P: Human tonsil, E14.5 mouse cerebral cortex and E14.5 rat cerebral cortex tissues. ICC/IF: NK-92 cells. IHC-Fr: Mouse E14.5 cerebrum and Rat E14.5 cerebrum tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.

 

 

IHC-Fr 1/100.

 

 

ICC/IF 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
    • Tissue specificity

      Expressed in CD8+ T-cells.
    • Involvement in disease

      Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.
    • Sequence similarities

      Contains 1 T-box DNA-binding domain.
    • Developmental stage

      Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • eomes antibody
      • EOMES_HUMAN antibody
      • Eomesodermin antibody
      • Eomesodermin homolog antibody
      • t box brain 2 antibody
      • T-box brain protein 2 antibody
      • T-brain-2 antibody
      • Tbr 2 antibody
      • TBR-2 antibody
      see all

    Images

    • Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat E14.5 cerebrum tissue labeling EOMES with ab216870 at 1/100 (5.75 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining in rat embryonic cerebrum (PMID: 24223221) is observed.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse E14.5 cerebrum tissue stained for EOMES using ab216870 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was  ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Positive staining in mouse embryonic cerebrum (PMID: 24223221) is observed. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labelling EOMES with ab216870 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NK-92 cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Immunohistochemical analysis of paraffin-embedded E14.5 rat cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 rat cerebral cortex (PMID: 18725516) is observed.  The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded E14.5 mouse cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 mouse cerebral cortex (PMID: 18725516) The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human tonsil is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    References

    ab216870 has not yet been referenced specifically in any publications.

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