Overview

  • Product name

    Anti-TBX1 antibody [EPR3289(2)]
    See all TBX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3289(2)] to TBX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TBX1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • PC12, F9, Neuro-2a, Jurkat and NCCIT cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.
    • Involvement in disease

      Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
      Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
      Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
      Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies.
    • Sequence similarities

      Contains 1 T-box DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Brachyury antibody
      • CAFS antibody
      • CTHM antibody
      • DGCR antibody
      • DGS antibody
      • DORV antibody
      • T box antibody
      • T box 1 antibody
      • T box 1 transcription factor antibody
      • T box 1 transcription factor C antibody
      • T box protein 1 antibody
      • T box transcription factor TBX 1 antibody
      • T box transcription factor TBX1 antibody
      • T-box protein 1 antibody
      • T-box transcription factor TBX1 antibody
      • TBX 1 antibody
      • TBX 1C antibody
      • tbx1 antibody
      • TBX1_HUMAN antibody
      • TBX1C antibody
      • Testis specific T box protein antibody
      • Testis-specific T-box protein antibody
      • TGA antibody
      • VCFS antibody
      see all

    Images

    • All lanes : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/1000 dilution

      Lane 1 : PC12 lysate
      Lane 2 : F9 lysate
      Lane 3 : Neuro-2a lysate
      Lane 4 : Jurkat lysate
      Lane 5 : NCCIT lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa
      Observed band size: 43 kDa

    References

    This product has been referenced in:

    • Velazquez-Villegas LA  et al. TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue. Nat Commun 9:245 (2018). Read more (PubMed: 29339725) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    This antibody recognizes an epitope within AA 90-120.

    Read More

    Answer

    Thank you for contacting me and for keeping me updated about the results with the Tbx1 and Twist antibodies. I'm sorry to hear that the results are not good at this point, but please let me know if there is anything I can do to help in that situation.

    Regarding the CYP26C1 antibody, I will get in touch with the lab to enquire about possible chick reactivity, as I don't have the immunogen sequence in my notes. I was only able to find a partial sequence for the chick CYP26C1 sequence-

    http://www.uniprot.org/uniprot/F1NZV9

    Do you have access to a full sequence?

    I will be in touch once I have more information from the lab, and I look forward to hearing from you.

    Have a good day.

    Read More

    Question
    Answer

    Thank you for your call today. Please see below for your testing codes, and let me know if you have any questions or need anything else from me.

    DISCOUNT CODES:
    Ab109313: ***
    Ab49254: ***

    Expiration date: August 28, 2012.

    These codes will eachgive you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in IF with chick samples,and include the correspondingcode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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