• Product name

  • Description

    Rabbit polyclonal to Tbx3
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide corresponding to Human Tbx3 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab111804)

  • Positive control

    • This antibody gave a positive signal in nuclear extracts from the following cell lines: HepG2; MCF7. IHC-P (FFPE): Human Placenta (Normal) tissue.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab99302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.



  • All lanes : Anti-Tbx3 antibody (ab99302) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 2 : MCF7 nuclear extract lysate (ab14860)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 79 kDa
    Observed band size: 79 kDa
    Additional bands at: 22 kDa, 77 kDa (possible isoform). We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes

    Ab99302 should recognise all three isoforms of Tbx3. We are probably observing Isoform I and Isoform II on this Western Blot image. The molecular weights of Isoform I and Isoform II are 77- and 79-kDa respectively.
  • ICC/IF image of ab99302 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99302 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HepG2 cells at 5ug/ml.

  • IHC image of Tbx3 staining in Human Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99302, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ab99302 staining Tbx3 in the AR42J-B13 rat pancreatic tumor cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton/PBS and blocked with blocking buffer for 60 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/5000.

    See Abreview


This product has been referenced in:

  • Bhattacharyya S  et al. Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition. Sci Rep 9:2106 (2019). Read more (PubMed: 30765799) »
  • Ichijo R  et al. Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration. Nat Commun 8:508 (2017). Read more (PubMed: 28894084) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Mouse Cell lysate - whole cell (small intestine)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
5 µg
BMP4 4h/6h/8h/12h
small intestine
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 28 2018

Immunocytochemistry/ Immunofluorescence
Rat Cell (AR42J-B13 Pancreatic tumor cell line)
Yes - 0.1% triton/PBS
AR42J-B13 Pancreatic tumor cell line
Blocking step
Roche Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Dr. Zoe Burke

Verified customer

Submitted May 12 2016

Western blot
Mouse Tissue lysate - other (E11.5 embryonic limb bud)
Loading amount
10 µg
E11.5 embryonic limb bud
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 03 2011

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