Product nameAnti-Tbx3 antibody [EPR10366(B)]
See all Tbx3 primary antibodies
DescriptionRabbit monoclonal [EPR10366(B)] to Tbx3
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues near the C terminus of Human Tbx3 (UniProt O15119).
- MCF7, HepG2 and 293T cell lysates; MCF7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 79 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTranscriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in TBX3 are the cause of ulnar-mammary syndrome (UMS) [MIM:181450]. UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands.
Sequence similaritiesContains 1 T-box DNA-binding domain.
- Information by UniProt
- Bladder cancer related protein XHL antibody
- T box 3 antibody
- T-box protein 3 antibody
All lanes : Anti-Tbx3 antibody [EPR10366(B)] (ab154828) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 79 kDa
Flow cytometric analysis of permeabilized 293T cells labeling Tbx3 with ab154828 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
This product has been referenced in:
- Peng J et al. The detrimental effects of glucocorticoids exposure during pregnancy on offspring's cardiac functions mediated by hypermethylation of bone morphogenetic protein-4. Cell Death Dis 9:834 (2018). Read more (PubMed: 30082698) »