Overview

  • Product name

  • Description

    Rabbit polyclonal to TBX5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-243 of Human TBX5 (UniProt ID: Q99593).

  • Positive control

    • HeLa and HepG2 whole cell lysate (ab7900); HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab137833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-TBX5 antibody (ab137833) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TBX5 using ab137833 at a 1/500 dilution.
    Lower image is costained with Hoechst 33342.

References

ab137833 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Limb Tissue)
Permeabilization
Yes - 1% Triton X-100
Specification
Limb Tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde

Gufa Lin

Verified customer

Submitted Apr 10 2017

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Embryos)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Treatment
hydroxyurea
Specification
Embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Nazem El Husseini

Verified customer

Submitted May 16 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocytes)
Specification
Human pluripotent stem cell derived cardiomyocytes
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 28 2014

Application
IHC - Wholemount
Sample
Mouse Tissue (E12.5 Sternum)
Specification
E12.5 Sternum

Ms. Sana Zakaria

Verified customer

Submitted Jan 28 2013

Answer

Thank you for contacting us. At this time, we do not have any feedback regarding the use of ab137833 on wholemount IHC. Unfortunately, we do not provide small or trial sized samples of any of our products. Under our new Abtrial program (which has replaced the 100% testing discount), the following discount code will give you a value off of a future order and can be redeemed for any product type.
DISCOUNT CODE: ***
Expiration date: February12th, 2013
Value: £190.00
I am very pleased to hear you would like to accept our offer and test ab137833 in IHC wholemount. This code will give you a discount off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC wholemount and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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