Anti-TBX5 antibody (ab223760)
Key features and details
- Rabbit polyclonal to TBX5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TBX5 antibody
See all TBX5 primary antibodies -
Description
Rabbit polyclonal to TBX5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human TBX5 aa 200-400.
Database link: Q99593 -
Positive control
- IHC-P: Human heart muscle, lung and placenta tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. -
Involvement in disease
Defects in TBX5 are the cause of Holt-Oram syndrome (HOS) [MIM:142900]. HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6910 Human
- Entrez Gene: 21388 Mouse
- Entrez Gene: 304514 Rat
- Omim: 601620 Human
- SwissProt: Q99593 Human
- SwissProt: P70326 Mouse
- SwissProt: Q5CZX7 Mouse
- SwissProt: Q5I2P1 Rat
see all -
Alternative names
- Holt Oram syndrome antibody
- HOS antibody
- T box 5 antibody
see all
Images
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Paraffin embedded sections of human heart muscle tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.
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Paraffin embedded sections of human lung tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.
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Paraffin embedded sections of human placenta tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.
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Paraffin embedded sections of human heart pancreas tissue were stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.
Negative control: no staining observed.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223760 has not yet been referenced specifically in any publications.