Product nameAnti-Tbx6 antibody
See all Tbx6 primary antibodies
DescriptionRabbit polyclonal to Tbx6
Tested applicationsSuitable for: IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Mouse Tbx6 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human and mouse testis and lung tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab38883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 25157815|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).|
FunctionProbable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.
Tissue specificityExpressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.
Sequence similaritiesContains 1 T-box DNA-binding domain.
Developmental stageExpressed during gastrulation and during a second phase in some adult tissues.
- Information by UniProt
- DFNB 67 antibody
- DFNB67 antibody
- SCDO5 antibody
All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Lung (Mouse) Tissue Lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
IHC-P image of Tbx6 staining with ab38883 on tissue sections from an E8.5 mouse embryo. The sections were Paraformaldehyde fixed, and permeabilized in PBST (PBS/0.5% v/v TritonX100), before incubation with ab38883 (1/500 dilution) for 48 hours at 4°C in 1% BSA, 5% donkey serum in PBST. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-rabbit polyclonal, at a 1/1000 dilution.
This product has been referenced in:
- Liu C et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). Read more (PubMed: 30008328) »
- Chal J et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). Read more (PubMed: 27583644) »