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Rabbit polyclonal to Tbx6
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Tbx6.
(Peptide available as
This antibody gave a positive signal in human and mouse testis and lung tissue lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 25157815
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.
Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.
Contains 1 T-box DNA-binding domain.
Expressed during gastrulation and during a second phase in some adult tissues.
Information by UniProt
DFNB 67 antibody
Western blot - Anti-Tbx6 antibody (ab38883)
All lanes :
Anti-Tbx6 antibody (ab38883) at 1 µg/ml
Lane 1 :
Testis (Mouse) Tissue Lysate
Lane 2 :
Lung (Mouse) Tissue Lysate
Lane 3 :
Human testis tissue lysate - total protein (
Lane 4 :
Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
50 kDa (
why is the actual band size different from the predicted?
Additional bands at:
43 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - Anti-Tbx6 antibody (ab38883) This image is courtesy of an anonymous abreview.
IHC-P image of Tbx6 staining with ab38883 on tissue sections from an E8.5 mouse embryo. The sections were Paraformaldehyde fixed, and permeabilized in PBST (PBS/0.5% v/v TritonX100), before incubation with ab38883 (1/500 dilution) for 48 hours at 4°C in 1% BSA, 5% donkey serum in PBST. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-rabbit polyclonal, at a 1/1000 dilution.
This product has been referenced in:
et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016).
Read more (PubMed: 27583644) »
et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014).
Read more (PubMed: 25157815) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"