Overview

  • Product name

  • Description

    Rabbit polyclonal to Tbx6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Tbx6 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab26243)

  • Positive control

    • This antibody gave a positive signal in human and mouse testis and lung tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab38883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 25157815
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).

Target

  • Function

    Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.
  • Tissue specificity

    Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Developmental stage

    Expressed during gastrulation and during a second phase in some adult tissues.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DFNB 67 antibody
    • DFNB67 antibody
    • SCDO5 antibody
    • T box 6 antibody
    • T box protein 6 antibody
    • T box transcription factor TBX 6 antibody
    • T box transcription factor TBX6 antibody
    • T-box protein 6 antibody
    • T-box transcription factor TBX6 antibody
    • Tbox 6 antibody
    • TBX 6 antibody
    • tbx6 antibody
    • TBX6_HUMAN antibody
    see all

Images

  • All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • IHC-P image of Tbx6 staining with ab38883 on tissue sections from an E8.5 mouse embryo. The sections were Paraformaldehyde fixed, and permeabilized in PBST (PBS/0.5% v/v TritonX100), before incubation with ab38883 (1/500 dilution) for 48 hours at 4°C in 1% BSA, 5% donkey serum in PBST. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-rabbit polyclonal, at a 1/1000 dilution.

    See Abreview

References

This product has been referenced in:

  • Liu C  et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). Read more (PubMed: 30008328) »
  • Chal J  et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). Read more (PubMed: 27583644) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Paraxial mesoderm)
Permeabilization
Yes - PBS/0.1% v/v TritonX100 (Sigma)
Specification
Paraxial mesoderm
Blocking step
FBS as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 13 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E8.5 mouse embryo section)
Specification
E8.5 mouse embryo section
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS/0.5% v/v TritonX100 (Sigma)
Blocking step
(agent) for 30 minute(s) · Concentration: 100%

Abcam user community

Verified customer

Submitted Aug 28 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (tail bud e12.5)
Loading amount
5 µg
Specification
tail bud e12.5
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 15 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up