Overview

  • Product name

  • Description

    Rabbit polyclonal to TC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human TC1 (internal sequence). The exact sequence is proprietary.
    Database link: O60779

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse liver tissue extract. ICC/IF: HeLa cells.
  • General notes

    Protein previously labeled as SLC19A2.

Properties

Applications

Our Abpromise guarantee covers the use of ab229680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.

Target

  • Function

    High-affinity transporter for the intake of thiamine.
  • Tissue specificity

    Ubiquitous; most abundant in skeletal and cardiac muscle. Medium expression in placenta, heart, liver and kidney, low in lung.
  • Involvement in disease

    Thiamine-responsive megaloblastic anemia syndrome
  • Sequence similarities

    Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • S19A2_HUMAN antibody
    • SLC19A2 antibody
    • Solute carrier family 19 member 2 antibody
    • TC1 antibody
    • Thiamine carrier 1 antibody
    • Thiamine transporter 1 antibody
    • THT1 antibody
    • ThTr 1 antibody
    • ThTr-1 antibody
    • ThTr1 antibody
    • TRMA antibody
    see all

Images

  • All lanes : Anti-TC1 antibody (ab229680) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 55 kDa



    10% SDS-PAGE gel.

  • Anti-TC1 antibody (ab229680) at 1/1000 dilution + Mouse liver tissue extract at 50 µg

    Predicted band size: 55 kDa



    10% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human  epithelial cell line from cervix adenocarcinoma) cells stained for TC1 (green) using ab229680 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229680 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229680.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up