Anti-TC10 antibody (ab168645)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TC10 antibody
See all TC10 primary antibodies -
DescriptionMouse polyclonal to TC10
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Full length protein corresponding to amino acids 1-205 of Human TC10 (NP_036381.2).
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Positive control
- TC10 transfected 293T cell lysate. HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab168645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.
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Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
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Post-translational
modificationsMay be post-translationally modified by both palmitoylation and polyisoprenylation. -
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 23433 Human
- Entrez Gene: 104215 Mouse
- Entrez Gene: 85428 Rat
- Omim: 605857 Human
- SwissProt: P17081 Human
- SwissProt: Q8R527 Mouse
- SwissProt: Q9JJL4 Rat
- Unigene: 709193 Human
see all -
Alternative names
- ARHQ antibody
- Ras homolog gene family member Q antibody
- RAS like family 7 member A antibody
see all
Images
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Western blot - Anti-TC10 antibody (ab168645)All lanes : Anti-TC10 antibody (ab168645) at 1 µg/ml
Lane 1 : TC10 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TC10 antibody (ab168645)Immunofluorescence analysis of HeLa cells, labeling TC10 with ab168645 at 10 µg/ml.
References
This product has been referenced in:
- Mayer S et al. Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission. Proc Natl Acad Sci U S A 110:20795-800 (2013). Read more (PubMed: 24297911) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"