Recombinant Anti-TC10 antibody [Y304] (ab32079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y304] to TC10
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TC10 antibody [Y304]
See all TC10 primary antibodies -
Description
Rabbit monoclonal [Y304] to TC10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human TC10 aa 100-200. The exact sequence is proprietary.
(Peptide available asab186757) -
Positive control
- 293 cell lysate, HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y304 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.Can be blocked with TC10 peptide (ab186757).
|
ICC/IF |
1/250 - 1/500.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.Can be blocked with TC10 peptide (ab186757). |
ICC/IF
1/250 - 1/500. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Post-translational
modificationsMay be post-translationally modified by both palmitoylation and polyisoprenylation. -
Cellular localization
Cytoplasm. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 23433 Human
- Entrez Gene: 85428 Rat
- Omim: 605857 Human
- SwissProt: P17081 Human
- SwissProt: Q9JJL4 Rat
- Unigene: 709193 Human
- Unigene: 4169 Rat
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Alternative names
- ARHQ antibody
- Ras homolog gene family member Q antibody
- RAS like family 7 member A antibody
see all
Images
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Anti-TC10 antibody [Y304] (ab32079) at 1/1000 dilution + 293 cell lysate
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of TC10 expression in HeLa cells using 1/250 ab 32079.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab32079 has been referenced in 5 publications.
- Liang J et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). PubMed: 29670091
- Fujita A et al. GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PLoS One 8:e79689 (2013). ICC/IF . PubMed: 24223996
- Heasman SJ et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). WB ; Human . PubMed: 20733052
- Fausther-Bovendo H et al. HIV gp41 engages gC1qR on CD4+ T cells to induce the expression of an NK ligand through the PIP3/H2O2 pathway. PLoS Pathog 6:e1000975 (2010). WB ; Human . PubMed: 20617170
- Yasuda S et al. An essential role of Cdc42-like GTPases in mitosis of HeLa cells. FEBS Lett 580:3375-80 (2006). PubMed: 16716304