Overview

  • Product name
    Anti-TC10 antibody [Y304]
    See all TC10 primary antibodies
  • Description
    Rabbit monoclonal [Y304] to TC10
  • Host species
    Rabbit
  • Specificity
    ab32079 recognises TC10.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human TC10 aa 100-200. The exact sequence is proprietary.
    (Peptide available as ab186757)

  • Positive control
    • 293 cell lysate, HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.Can be blocked with TC10 peptide (ab186757).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.
    • Sequence similarities
      Belongs to the small GTPase superfamily. Rho family.
    • Post-translational
      modifications
      May be post-translationally modified by both palmitoylation and polyisoprenylation.
    • Cellular localization
      Cytoplasm. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHQ antibody
      • Ras homolog gene family member Q antibody
      • RAS like family 7 member A antibody
      • Ras like protein family member 7A antibody
      • Ras like protein TC10 antibody
      • Ras related GTP binding protein TC10 antibody
      • Ras-like protein family member 7A antibody
      • Ras-like protein TC10 antibody
      • RASL7A antibody
      • Rho related GTP binding protein RhoQ antibody
      • Rho-related GTP-binding protein RhoQ antibody
      • Rhoq antibody
      • RHOQ_HUMAN antibody
      • TC10A antibody
      see all

    Images

    • Anti-TC10 antibody [Y304] (ab32079) at 1/1000 dilution + 293 cell lysate

      Predicted band size: 23 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?

    • Immunofluorescent analysis of TC10 expression in HeLa cells using 1/250 ab 32079.

    References

    This product has been referenced in:
    • Liang J  et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). Read more (PubMed: 29670091) »
    • Fujita A  et al. GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PLoS One 8:e79689 (2013). ICC/IF . Read more (PubMed: 24223996) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

    Credit ID: ####


    As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

    I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

    Read More

    Answer

    Thank you fo ryour reply and for confirming these details.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I would still suggest it would be beneficial to considertrying the experiment again with a suitable endogenous positive control, such as HeLa or293 cells, as previously discussed. So you are detecting the native protein and not a recombinant protein.

    However, as an exception in this case, I am pleased to offer you a free of charge replacement or credit note in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Answer

    Thank you for taking the time to provide these details. Theinformation you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this case, I would like to offer some suggestions to help optimise the results. I would also appreciate if you can confirm some further details:

    1. There are inherent difficulties with antibody detection of recombinant proteins that I can recommend considering, particularly if they are tagged. This may be a biological artifact rather than a problem with the antibody.Could you confirm that the protein transfected is full length and includes the immunogen sequence? Also, if the recombinant TC10 is tagged, it is possible that the tag is preventing access to the antibody. Could you confirm at which region of the protein the tag has been placed?

    2. Could you confirm the species from which the recombinant TC10 gene was taken?

    3. Could you confirm how the stability of transfection has been assessed?

    4. I can recommend that due to the points described above, it is important to use an endogenous positive control sample. For example, this antibody has been used successfully on293 cell lysate.

    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results with an endogenous positive control, please do not hesitate to contact me again with the further requested details.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (embryonic dorsal root ganglia)
    Loading amount
    30 µg
    Specification
    embryonic dorsal root ganglia
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

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    Verified customer

    Submitted Nov 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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