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Here is the customer reply:
'The endogenous control sample was HEK293T cell lysate created using pEF plasmid. Marking with anti TC10 showed no band, marking with anti-flag produced a band.'
Thank you for the kind support and a good week to you.
Asked on Sep 03 2012
Thank you fo ryour reply and for confirming these details.
I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I would still suggest it would be beneficial to considertrying the experiment again with a suitable endogenous positive control, such as HeLa or293 cells, as previously discussed. So you are detecting the native protein and not a recombinant protein.
However, as an exception in this case, I am pleased to offer you a free of charge replacement or credit note in compensation.
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.
Answered on Sep 03 2012