Anti-TC21 antibody [EM-50] (ab234145)
Key features and details
- Mouse monoclonal [EM-50] to TC21
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-TC21 antibody [EM-50]
See all TC21 primary antibodies -
Description
Mouse monoclonal [EM-50] to TC21 -
Host species
Mouse -
Specificity
This antibody does not react with R-Ras1, H-Ras, K-Ras and N-Ras. -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human TC21.
Database link: P62070 -
Positive control
- ICC/IF: TC21 transfected COS cells. Flow Cyt (Intra): TC21-GFP transfected HEK-293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
EM-50 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion. -
Tissue specificity
Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas. -
Involvement in disease
Ovarian cancer -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Post-translational
modificationsMay be post-translationally modified by both palmitoylation and polyisoprenylation. -
Cellular localization
Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS). - Information by UniProt
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Database links
- Entrez Gene: 22800 Human
- Omim: 600098 Human
- SwissProt: P62070 Human
- Unigene: 502004 Human
- Unigene: 712835 Human
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Alternative names
- C86394 antibody
- Oncogene RRAS2 antibody
- Oncogene TC21 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234145 has not yet been referenced specifically in any publications.